@article{e8e8e73a76284f4eb1e63a4e03a9381b,
title = "Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine",
abstract = "O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that regulates normal physiology and the cell stress response. Dysregulation of O-GlcNAc cycling is implicated in the etiology of type II diabetes, heart failure, hypertension, and Alzheimer's disease, as well as cardioprotection. These protocols cover simple and comprehensive techniques for detecting proteins modified by O-GlcNAc and studying the enzymes that add or remove O-GlcNAc.",
keywords = "O-GlcNAc, O-linked, analysis, detection, galactosyltransferase, glycosylation, signal transduction",
author = "Kamau Fahie and Bhargavi Narayanan and Fiddia Zahra and Russell Reeves and Fernandes, {Steve M.} and Hart, {Gerald W.} and Zachara, {Natasha E.}",
note = "Funding Information: Original research in the authors{\textquoteright} laboratories was supported by the U.S. National Institutes of Health (K12HL141952, U01 CA230978, and RO1HL139640 to N.E.Z.). G.W.H. receives a percentage of royalties received by the university on sales of the CTD 110.6 antibody. The terms of these arrangements are in accordance with the conflict of interest policy at The Johns Hopkins University. Funding Information: Original research in the authors{\textquoteright} laboratories was supported by the U.S. National Institutes of Health (K12HL141952, U01 CA230978, and RO1HL139640 to N.E.Z.). G.W.H. receives a percentage of royalties received by the university on sales of the CTD 110.6 antibody. The terms of these arrangements are in accordance with the conflict of interest policy at The Johns Hopkins University. Publisher Copyright: {\textcopyright} 2021 The Authors. Current Protocols published by Wiley Periodicals LLC.",
year = "2021",
month = may,
doi = "10.1002/cpz1.129",
language = "English (US)",
volume = "1",
journal = "Current Protocols",
issn = "2691-1299",
publisher = "John Wiley & Sons Inc.",
number = "5",
}