Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Natasha E. Zachara, Gerald W. Hart, Robert N. Cole, Yuan Gao

Research output: Contribution to journalArticlepeer-review

Abstract

The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

Original languageEnglish (US)
Pages (from-to)Unit 17.6
JournalCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
VolumeChapter 17
DOIs
StatePublished - Feb 2002

ASJC Scopus subject areas

  • Molecular Biology

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