Detection and analysis of proteins modified by O-Linked N-Acetylglucosamine

Natasha E. Zachara; Keith Vosseller; Gerald W. Hart

doi:10.1002/0471140864.ps1208s66

Detection and analysis of proteins modified by O-Linked N-Acetylglucosamine

Natasha E. Zachara, Keith Vosseller, Gerald W. Hart

School of Medicine

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, andmapping O-GlcNAc modification sites.

Original language	English (US)
Article number	12.8
Journal	Current Protocols in Protein Science
Volume	1
Issue number	SUPPL.66
DOIs	https://doi.org/10.1002/0471140864.ps1208s66
State	Published - Nov 2011

Keywords

Analysis
Galactosyltransferase
Glycosylation
Method(s)
O-glcnac
O-linked
Signal transduction
detection

ASJC Scopus subject areas

Structural Biology
Biochemistry

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10.1002/0471140864.ps1208s66

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KW - Analysis

KW - Galactosyltransferase

KW - Glycosylation

KW - Method(s)

KW - O-glcnac

KW - O-linked

KW - Signal transduction

KW - detection

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Detection and analysis of proteins modified by O-Linked N-Acetylglucosamine

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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