Detection and analysis of proteins modified by O-Linked N-Acetylglucosamine

Natasha E Zachara, Keith Vosseller, Gerald Warren Hart

Research output: Contribution to journalArticle

Abstract

O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, andmapping O-GlcNAc modification sites.

Original languageEnglish (US)
Article number12.8
JournalCurrent Protocols in Protein Science
Volume1
Issue numberSUPPL.66
DOIs
StatePublished - Nov 2011

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Keywords

  • Analysis
  • detection
  • Galactosyltransferase
  • Glycosylation
  • Method(s)
  • O-glcnac
  • O-linked
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology

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