Abstract
O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, andmapping O-GlcNAc modification sites.
Original language | English (US) |
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Article number | 12.8 |
Journal | Current Protocols in Protein Science |
Volume | 1 |
Issue number | SUPPL.66 |
DOIs | |
State | Published - Nov 2011 |
Keywords
- Analysis
- Galactosyltransferase
- Glycosylation
- Method(s)
- O-glcnac
- O-linked
- Signal transduction
- detection
ASJC Scopus subject areas
- Structural Biology
- Biochemistry