Detection and analysis of proteins modified by O-Linked N-Acetylglucosamine

Natasha E. Zachara, Keith Vosseller, Gerald W. Hart

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, andmapping O-GlcNAc modification sites.

Original languageEnglish (US)
Article number12.8
JournalCurrent Protocols in Protein Science
Issue numberSUPPL.66
StatePublished - Nov 2011


  • Analysis
  • Galactosyltransferase
  • Glycosylation
  • Method(s)
  • O-glcnac
  • O-linked
  • Signal transduction
  • detection

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry


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