Detection and analysis of proteins modified by O-linked N-acetylglucosamine

Natasha E. Zachara, Keith Vosseller, Gerald W. Hart

Research output: Contribution to journalArticle


O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.

Original languageEnglish (US)
JournalCurrent protocols in molecular biology
Issue numberSUPPL. 95
StatePublished - Aug 29 2011


  • Analysis
  • Detection
  • Galactosyltransferase
  • Glycosylation
  • Method(s)
  • O-GlcNAc
  • O-linked
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Biology

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