Detection and analysis of proteins modified by O-linked N-acetylglucosamine

Natasha E. Zachara; Keith Vosseller; Gerald W. Hart

doi:10.1002/0471142727.mb1706s95

Detection and analysis of proteins modified by O-linked N-acetylglucosamine

Natasha E. Zachara, Keith Vosseller, Gerald W. Hart

School of Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.

Original language	English (US)
Journal	Current protocols in molecular biology
Issue number	SUPPL. 95
DOIs	https://doi.org/10.1002/0471142727.mb1706s95
State	Published - 2011

Keywords

Analysis
Detection
Galactosyltransferase
Glycosylation
Method(s)
O-GlcNAc
O-linked
Signal transduction

ASJC Scopus subject areas

Molecular Biology

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10.1002/0471142727.mb1706s95

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abstract = "O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.",

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AU - Zachara, Natasha E.

AU - Vosseller, Keith

AU - Hart, Gerald W.

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N2 - O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.

AB - O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.

KW - Analysis

KW - Detection

KW - Galactosyltransferase

KW - Glycosylation

KW - Method(s)

KW - O-GlcNAc

KW - O-linked

KW - Signal transduction

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JO - Current protocols in molecular biology

JF - Current protocols in molecular biology

IS - SUPPL. 95

ER -

Detection and analysis of proteins modified by O-linked N-acetylglucosamine

Abstract

Keywords

ASJC Scopus subject areas

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