Detecting significant changes in protein abundance

Research output: Contribution to journalArticlepeer-review

Abstract

We review and demonstrate how an empirical Bayes method, shrinking a protein's sample variance towards a pooled estimate, leads to far more powerful and stable inference to detect significant changes in protein abundance compared to ordinary t-tests. Using examples from isobaric mass labelled proteomic experiments we show how to analyze data from multiple experiments simultaneously, and discuss the effects of missing data on the inference. We also present easy to use open source software for normalization of mass spectrometry data and inference based on moderated test statistics.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalEuPA Open Proteomics
Volume7
DOIs
StatePublished - Jun 1 2015

Keywords

  • Empirical Bayes
  • Inference
  • Protein abundance

ASJC Scopus subject areas

  • Biochemistry

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