Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications

Carrie Baker Brachmann, Adrian Davies, Gregory J. Cost, Emerita Caputo, Joachim Li, Philip Hieter, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes.

Original languageEnglish (US)
Pages (from-to)115-132
Number of pages18
JournalYeast
Volume14
Issue number2
DOIs
StatePublished - Jan 30 1998

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gene targeting
Yeast
Saccharomyces cerevisiae
plasmids
Plasmids
Genes
Polymerase Chain Reaction
genetic markers
yeasts
Yeasts
molecular genetics
genomics
Molecular Biology
gene expression
genome
Gene expression
Genome
Gene Expression
genes

Keywords

  • Auxotrophic markers
  • Bacteria-yeast shuttle vectors
  • Gene disruption
  • S288C
  • Saccharomyces cerevisiae
  • Yeast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Designer deletion strains derived from Saccharomyces cerevisiae S288C : A useful set of strains and plasmids for PCR-mediated gene disruption and other applications. / Brachmann, Carrie Baker; Davies, Adrian; Cost, Gregory J.; Caputo, Emerita; Li, Joachim; Hieter, Philip; Boeke, Jef D.

In: Yeast, Vol. 14, No. 2, 30.01.1998, p. 115-132.

Research output: Contribution to journalArticle

Brachmann, Carrie Baker ; Davies, Adrian ; Cost, Gregory J. ; Caputo, Emerita ; Li, Joachim ; Hieter, Philip ; Boeke, Jef D. / Designer deletion strains derived from Saccharomyces cerevisiae S288C : A useful set of strains and plasmids for PCR-mediated gene disruption and other applications. In: Yeast. 1998 ; Vol. 14, No. 2. pp. 115-132.
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