Design of composite inhibitors targeting glutamate carboxypeptidase II: The importance of effector functionalities

Zora Novakova, Jiri Cerny, Cindy J. Choy, Jessie R. Nedrow, Joeseph K. Choi, Jacek Lubkowski, Clifford E. Berkman, Cyril Barinka

Research output: Contribution to journalArticle

Abstract

Inhibitors targeting human glutamate carboxypeptidase II (GCPII) typically consist of a P1′ glutamate-derived binding module, which warrants the high affinity and specificity, linked to an effector function that is positioned within the entrance funnel of the enzyme. Here we present a comprehensive structural and computational study aimed at dissecting the importance of the effector function for GCPII binding and affinity. To this end we determined crystal structures of human GCPII in complex with a series of phosphoramidate-based inhibitors harboring effector functions of diverse physicochemical characteristics. Our data show that higher binding affinities of phosphoramidates, compared to matching phosphonates, are linked to the presence of additional hydrogen bonds between Glu424 and Gly518 of the enzyme and the amide group of the phosphoramidate. While the positioning of the P1′ glutamate-derived module within the S1′ pocket of GCPII is invariant, interaction interfaces between effector functions and residues lining the entrance funnel are highly varied, with the positively charged arginine patch defined by Arg463, Arg534 and Arg536 being the only 'hot-spot' common to several studied complexes. This variability stems in part from the fact that the effector/GCPII interfaces generally encompass isolated areas of nonpolar residues within the entrance funnel and resulting van der Waals contacts lack the directionality typical for hydrogen bonding interactions. The presented data unravel a complexity of binding modes of inhibitors within non-prime site(s) of GCPII and can be exploited for the design of novel GCPII-specific compounds.

Original languageEnglish (US)
Pages (from-to)130-143
Number of pages14
JournalFEBS Journal
Volume283
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Keywords

  • NAALADase
  • X-ray crystallography
  • molecular modeling
  • phosphoramidate
  • prostate-specific membrane antigen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Novakova, Z., Cerny, J., Choy, C. J., Nedrow, J. R., Choi, J. K., Lubkowski, J., Berkman, C. E., & Barinka, C. (2016). Design of composite inhibitors targeting glutamate carboxypeptidase II: The importance of effector functionalities. FEBS Journal, 283(1), 130-143. https://doi.org/10.1111/febs.13557