TY - JOUR
T1 - Design of a synthetic yeast genome
AU - Richardson, Sarah M.
AU - Mitchell, Leslie A.
AU - Stracquadanio, Giovanni
AU - Yang, Kun
AU - Dymond, Jessica S.
AU - DiCarlo, James E.
AU - Lee, Dongwon
AU - Huang, Cheng Lai Victor
AU - Chandrasegaran, Srinivasan
AU - Cai, Yizhi
AU - Boeke, Jef D.
AU - Bader, Joel S.
N1 - Funding Information:
This work was supported in part by funding from NSF (grants MCB-0718846 and MCB-1026068 to J.D.B. and J.S.B., MCB-1616111 to J.D.B., MCB-0546446 and MCB-1445545 to J.S.B.), the Department of Energy (grant number DE-FG02097ER25308 to S.M.R.); NSERC Postdoctoral Fellowship to L.A.M.; and Microsoft Research (to J.S.B.). We thank all Sc2.0 Consortium members and members of the S. cerevisiae community for their helpful comments during the design phase of this project. We thank the leadership and staff of the SGD for helping make this project possible. J.D.B. and J.S.B. are founders and directors of Neochromosome, Inc. J.D.B. serves as a scientific advisor to Recombinetics, Inc., and Sample6, Inc. These arrangements are reviewed and managed by the committees on conflict of interest at NYU Langone Medical Center (J.D.B.) and Johns Hopkins University (J.S.B.). Additional information including design diagrams, PCRTag sequences, feature summary tables, and variants in the physical strains corresponding to Sc2.0 synthetic chromosomes can be accessed on the Sc2.0 website, www.syntheticyeast.org.
Publisher Copyright:
© 2017, American Association for the Advancement of Science. All rights reserved.
PY - 2017/3/10
Y1 - 2017/3/10
N2 - We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.
AB - We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.
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U2 - 10.1126/science.aaf4557
DO - 10.1126/science.aaf4557
M3 - Article
C2 - 28280199
AN - SCOPUS:85014805071
SN - 0036-8075
VL - 355
SP - 1040
EP - 1044
JO - Science
JF - Science
IS - 6329
ER -