Design of a synthetic yeast genome

Sarah M. Richardson, Leslie A. Mitchell, Giovanni Stracquadanio, Kun Yang, Jessica S. Dymond, James E. DiCarlo, Dongwon Lee, Cheng Lai Victor Huang, Srinivasan Chandrasegaran, Yizhi Cai, Jef D. Boeke, Joel S. Bader

Research output: Contribution to journalArticle

Abstract

We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.

Original languageEnglish (US)
Pages (from-to)1040-1044
Number of pages5
JournalScience
Volume355
Issue number6329
DOIs
StatePublished - Mar 10 2017

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Yeasts
Genome
Chromosomes
Endoreduplication
Chromosome Structures
Saccharomyces cerevisiae
Nucleotides

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Richardson, S. M., Mitchell, L. A., Stracquadanio, G., Yang, K., Dymond, J. S., DiCarlo, J. E., ... Bader, J. S. (2017). Design of a synthetic yeast genome. Science, 355(6329), 1040-1044. https://doi.org/10.1126/science.aaf4557

Design of a synthetic yeast genome. / Richardson, Sarah M.; Mitchell, Leslie A.; Stracquadanio, Giovanni; Yang, Kun; Dymond, Jessica S.; DiCarlo, James E.; Lee, Dongwon; Huang, Cheng Lai Victor; Chandrasegaran, Srinivasan; Cai, Yizhi; Boeke, Jef D.; Bader, Joel S.

In: Science, Vol. 355, No. 6329, 10.03.2017, p. 1040-1044.

Research output: Contribution to journalArticle

Richardson, SM, Mitchell, LA, Stracquadanio, G, Yang, K, Dymond, JS, DiCarlo, JE, Lee, D, Huang, CLV, Chandrasegaran, S, Cai, Y, Boeke, JD & Bader, JS 2017, 'Design of a synthetic yeast genome', Science, vol. 355, no. 6329, pp. 1040-1044. https://doi.org/10.1126/science.aaf4557
Richardson SM, Mitchell LA, Stracquadanio G, Yang K, Dymond JS, DiCarlo JE et al. Design of a synthetic yeast genome. Science. 2017 Mar 10;355(6329):1040-1044. https://doi.org/10.1126/science.aaf4557
Richardson, Sarah M. ; Mitchell, Leslie A. ; Stracquadanio, Giovanni ; Yang, Kun ; Dymond, Jessica S. ; DiCarlo, James E. ; Lee, Dongwon ; Huang, Cheng Lai Victor ; Chandrasegaran, Srinivasan ; Cai, Yizhi ; Boeke, Jef D. ; Bader, Joel S. / Design of a synthetic yeast genome. In: Science. 2017 ; Vol. 355, No. 6329. pp. 1040-1044.
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