TY - GEN
T1 - Design considerations for a miniature multicontrast neuroimager
AU - Senarathna, Janaka
AU - Wang, Qihong
AU - Tyler, Betty
AU - Thakor, Nitish
AU - Pathak, Arvind
N1 - Funding Information:
This research was funded by NCI grants 1R21CA175784-01 and 1R01CA196701, and a Kavli Neuroscience Discovery Institute Distinguished Postdoctoral Fellowship (JS). The authors wish to thank Mr. Jay Burns of the Johns Hopkins University School of Medicine Biomedical Engineering workshop for help with miniaturization considerations.
Publisher Copyright:
© 2019 SPIE.
PY - 2019
Y1 - 2019
N2 - Traditional optical imaging systems are designed for imaging with a single contrast mechanism, and therefore can interrogate only a single neurophysiologic variable. However, the biological complexity underlying neurophysiological function and its alteration in neurodegenerative diseases, requires the simultaneous interrogation of multiple neurophysiologic variables to arrive at a better understanding. Today's multicontrast optical imaging systems satisfy this need, but suffer from some inherent limitations. Owing to the need to integrate multiple contrast mechanisms, these imaging systems tend to be benchtop-based and unportable, often requiring animals to be anesthetized, custom built and expensive. This limits their widespread adoption. Miniaturization, although technically challenging, remains a potential solution to these limitations. To address this unmet need, here we present the design considerations and practical guidelines for building inexpensive, miniaturized, and portable multicontrast optical neuroimaging systems that allow comprehensive interrogation of brain function in freely behaving rodents. We then showcase an example tri-contrast miniature neuroimaging system and demonstrate the implementation of our guidelines. We conclude by demonstrating the utility of such a miniature multicontrast neuroimaging system by interrogating in an awake rodent the tumor extent, angiogenic vascular sprouting, flow establishment in the newly formed vessels, as well as anomalies in resting-state microvascular fluctuations in a preclinical model of brain tumor progression.
AB - Traditional optical imaging systems are designed for imaging with a single contrast mechanism, and therefore can interrogate only a single neurophysiologic variable. However, the biological complexity underlying neurophysiological function and its alteration in neurodegenerative diseases, requires the simultaneous interrogation of multiple neurophysiologic variables to arrive at a better understanding. Today's multicontrast optical imaging systems satisfy this need, but suffer from some inherent limitations. Owing to the need to integrate multiple contrast mechanisms, these imaging systems tend to be benchtop-based and unportable, often requiring animals to be anesthetized, custom built and expensive. This limits their widespread adoption. Miniaturization, although technically challenging, remains a potential solution to these limitations. To address this unmet need, here we present the design considerations and practical guidelines for building inexpensive, miniaturized, and portable multicontrast optical neuroimaging systems that allow comprehensive interrogation of brain function in freely behaving rodents. We then showcase an example tri-contrast miniature neuroimaging system and demonstrate the implementation of our guidelines. We conclude by demonstrating the utility of such a miniature multicontrast neuroimaging system by interrogating in an awake rodent the tumor extent, angiogenic vascular sprouting, flow establishment in the newly formed vessels, as well as anomalies in resting-state microvascular fluctuations in a preclinical model of brain tumor progression.
KW - Awake animal
KW - Fluorescence (FL)
KW - Imaging
KW - Intrinsic optical signals (IOS)
KW - Laser speckle contrast (LSC)
KW - Miniaturized
KW - Multicontrast imaging
KW - Optogenetics
KW - Tumor
UR - http://www.scopus.com/inward/record.url?scp=85064845524&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064845524&partnerID=8YFLogxK
U2 - 10.1117/12.2524622
DO - 10.1117/12.2524622
M3 - Conference contribution
AN - SCOPUS:85064845524
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Clinical and Translational Neurophotonics 2019
A2 - Madsen, Steen J.
A2 - Yang, Victor X. D.
A2 - Thakor, Nitish V.
PB - SPIE
T2 - Clinical and Translational Neurophotonics 2019
Y2 - 2 February 2019 through 3 February 2019
ER -