Design and fabrication of N-Alkyl-polyethylenimine-stabilized iron oxide nanoclusters for gene delivery

Gang Liu, Zhiyong Wang, Seulki Lee, Hua Ai, Xiaoyuan Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

With the rapid development of nanotechnology, inorganic magnetic nanoparticles, especially iron oxide nanoparticles (IOs), have emerged as great vehicles for biomedical diagnostic and therapeutic applications. In order to rationally design IO-based gene delivery nanovectors, surface modification is essential and determines the loading and release of the gene of interest. Here we highlight the basic concepts and applications of nonviral gene delivery vehicles based on low molecular weight N-alkyl polyethylenimine-stabilized IOs. The experimental protocols related to these topics are described in this chapter.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages263-276
Number of pages14
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume509
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Gene delivery
  • Polyethylenimine
  • Small interfering RNA
  • Superparamagnetic nanoparticles

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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