Design and Construction of Inducible Expression Vector by Single-Plasmid Strategy

Ding Xie Liu, Xiao Wei Zhou, Pei Tang Huang

Research output: Contribution to journalArticle

Abstract

On the basis of T-Rex inducible expression system, a vector that express reporter gene and regulated gene simultaneously were constructed. The induction effects of tetracycline to the gene expression were studied in CV1 cells by transient transfection. An 8.9-fold increase in expression level of reporter gene was observed when CV1 cells, which were transiently transfected with the vector, were induced with tetracycline for 24 h, suggesting that the application of single-plasmid vecor in gene expression induction is also feasible.

Original languageEnglish (US)
Pages (from-to)824-825
Number of pages2
JournalProgress in Biochemistry and Biophysics
Volume30
Issue number5
StatePublished - Oct 2003
Externally publishedYes

Keywords

  • Expression system
  • Single-plasmid strategy
  • Tetracycline
  • Vector

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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