Design-a-gene with genedesign

Sarah M. Richardson, Steffi Liu, Jef D. Boeke, Joel S. Bader

Research output: Chapter in Book/Report/Conference proceedingChapter


The manual design of synthetic genes is a tedious and error-prone process-even for very short genes-and it becomes completely infeasible when multiple synthetic genes are needed. GeneDesign is a set of modules that automate batch nucleotide manipulation. Here, we explain the installation, configuration, and use of GeneDesign as part of a synthetic design workflow.

Original languageEnglish (US)
Title of host publicationGene Synthesis
Subtitle of host publicationMethods and Protocols
EditorsJean Peccoud
Number of pages13
StatePublished - Mar 22 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745



  • Codon optimization
  • Computer-assisted GeneDesign
  • Synthetic biology
  • Synthetic biology software
  • Synthetic genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Richardson, S. M., Liu, S., Boeke, J. D., & Bader, J. S. (2012). Design-a-gene with genedesign. In J. Peccoud (Ed.), Gene Synthesis: Methods and Protocols (pp. 235-247). (Methods in Molecular Biology; Vol. 852).