Desensitization of the P2X2 receptor controlled by alternative splicing

U. Brändle, P. Spielmanns, R. Osteroth, J. Sim, A. Surprenant, G. Buell, J. P. Ruppersberg, P. K. Plinkert, H. P. Zenner, E. Glowatzki

Research output: Contribution to journalArticle

Abstract

P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X2 receptor splice variant (P2X2-2) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X2 gene. P2X2-2 is as highly expressed as the original P2X2 sequence in various tissues. ATP-activated currents mediated by heterologous expressed P2X2 or P2X2-2 receptors showed significant differences in desensitization time constants and steady-state currents in the continuous presence of ATP. These results imply functional differences between cells differentially expressing these P2X2 isoforms.

Original languageEnglish (US)
Pages (from-to)294-298
Number of pages5
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
StatePublished - Mar 10 1997
Externally publishedYes

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Keywords

  • Alternative splicing
  • Desensitization
  • Genomic sequence
  • P2X receptor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Brändle, U., Spielmanns, P., Osteroth, R., Sim, J., Surprenant, A., Buell, G., Ruppersberg, J. P., Plinkert, P. K., Zenner, H. P., & Glowatzki, E. (1997). Desensitization of the P2X2 receptor controlled by alternative splicing. FEBS Letters, 404(2-3), 294-298. https://doi.org/10.1016/S0014-5793(97)00128-2