Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler® PCR assay with inhibition control

Joann L. Cloud, Weston C. Hymas, Arthur Turlak, Ann Croft, Udo Reischl, Judy A. Daly, Karen C. Carroll

Research output: Contribution to journalArticlepeer-review

Abstract

While culture for Bordetella species is highly specific, sensitivity is extremely variable due to patient age, immunization status, antibiotic treatment, and specimen transport conditions. We evaluated a real-time multiplex PCR assay as an alternative to culture for the detection and differentiation of Bordetella pertussis and Bordetella parapertussis. The PCR conditions allowed the simultaneous detection of one B. pertussis organism and five B. parapertussis organisms per reaction. An inhibition control was incorporated into the assay. Of 163 total samples evaluated, 37 of 38 samples positive by either culture or direct fluorescent antibody testing (DFA) were also positive by PCR (97% sensitivity). Of 125 culture- or DFA-negative samples, 101 were also negative by PCR (81% specificity). The described multiplex assay is a rapid, sensitive, contamination-limiting, real-time PCR assay that controls for inhibition. The assay performs well using liquid or swab samples and from dried material on slides.

Original languageEnglish (US)
Pages (from-to)189-195
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume46
Issue number3
DOIs
StatePublished - Jul 1 2003

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler® PCR assay with inhibition control'. Together they form a unique fingerprint.

Cite this