Derivation of chondrogenically-committed cells from human embryonic cells for cartilage tissue regeneration

Nathaniel S. Hwang, Shyni Varghese, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Background: Heterogeneous and uncontrolled differentiation of human embryonic stem cells (hESCs) in embryoid bodies (EBs) limits the potential use of hESCs for cell-based therapies. More efficient strategies are needed for the commitment and differentiation of hESCs to produce a homogeneous population of specific cell types for tissue regeneration applications. Methodology/Principal Findings: We report here that significant chondrocytic commitment of feeder-free cultured human embryonic stem cells (FF-hESCs), as determined by gene expression and immunostaining analysis, was induced by co-culture with primary chondrocytes. Furthermore, a dynamic expression profile of chondrocyte-specific genes was observed during monolayer expansion of the chondrogenically-committed cells. Chondrogenically committed cells synergistically responded to transforming growth factor-β1 (TGF-β1) and β1-integrin activating antibody by increasing tissue mass in pellet culture. In addition, when encapsulated in hydrogels, these cells formed cartilage tissue both in vitro and in vivo. In contrast, the absence of chondrocyte co-culture did not result in an expandable cell population from FF-hESCs. Conclusions/Significance: The direct chondrocytic commitment of FF-hESCs can be induced by morphogenetic factors from chondrocytes without EB formation and homogenous cartilage tissue can be formed in vitro and in vivo.

Original languageEnglish (US)
Article numbere2498
JournalPLoS One
Volume3
Issue number6
DOIs
StatePublished - Jun 25 2008

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Tissue regeneration
chondrocytes
embryonic stem cells
Cartilage
tissue repair
Stem cells
Regeneration
Chondrocytes
Embryoid Bodies
coculture
cells
Tissue
Coculture Techniques
cartilage
Cells
Feeder Cells
transforming growth factors
Hydrogels
hydrocolloids
integrins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Derivation of chondrogenically-committed cells from human embryonic cells for cartilage tissue regeneration. / Hwang, Nathaniel S.; Varghese, Shyni; Elisseeff, Jennifer Hartt.

In: PLoS One, Vol. 3, No. 6, e2498, 25.06.2008.

Research output: Contribution to journalArticle

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