TY - JOUR
T1 - Deregulation of glucose transporter 1 and glycolytic gene expression by c-Myc
AU - Osthus, Rebecca C.
AU - Shim, Hyunsuk
AU - Kim, Sunkyu
AU - Li, Qing
AU - Reddy, Rahul
AU - Mukherjee, Mita
AU - Xu, Yi
AU - Wonsey, Diane
AU - Lee, Linda A.
AU - Dang, Chi V.
PY - 2000/7/21
Y1 - 2000/7/21
N2 - Unlike normal mammalian cells, which use oxygen to generate energy, cancer cells rely on glycolysis for energy and are therefore less dependent on oxygen. We previously observed that the c-Myc oncogenic transcription factor regulates lactate dehydrogenase A and induces lactate overproduction. We, therefore, sought to determine whether c-Myc controls other genes regulating glucose metabolism. In Rat1a fibroblasts and murine livers overexpressing c-Myc, the mRNA levels of the glucose transporter GLUT1, phosphoglucose isomerase, phosphofructokinase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and enolase were elevated. c-Myc directly transactivates genes encoding GLUT1, phosphofructokinase, and enolase and increases glucose uptake in Rat1 fibroblasts. Nuclear run-on studies confirmed that the GLUT1 transcriptional rate is elevated by c-Myc. Our findings suggest that overexpression of the c-Myc oncoprotein deregulates glycolysis through the activation of several components of the glucose metabolic pathway.
AB - Unlike normal mammalian cells, which use oxygen to generate energy, cancer cells rely on glycolysis for energy and are therefore less dependent on oxygen. We previously observed that the c-Myc oncogenic transcription factor regulates lactate dehydrogenase A and induces lactate overproduction. We, therefore, sought to determine whether c-Myc controls other genes regulating glucose metabolism. In Rat1a fibroblasts and murine livers overexpressing c-Myc, the mRNA levels of the glucose transporter GLUT1, phosphoglucose isomerase, phosphofructokinase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and enolase were elevated. c-Myc directly transactivates genes encoding GLUT1, phosphofructokinase, and enolase and increases glucose uptake in Rat1 fibroblasts. Nuclear run-on studies confirmed that the GLUT1 transcriptional rate is elevated by c-Myc. Our findings suggest that overexpression of the c-Myc oncoprotein deregulates glycolysis through the activation of several components of the glucose metabolic pathway.
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U2 - 10.1074/jbc.C000023200
DO - 10.1074/jbc.C000023200
M3 - Article
C2 - 10823814
AN - SCOPUS:0034698178
SN - 0021-9258
VL - 275
SP - 21797
EP - 21800
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 29
ER -