"Depupylation" of Prokaryotic Ubiquitin-like Protein from Mycobacterial Proteasome Substrates

Kristin E. Burns, Francisca A. Cerda-Maira, Tao Wang, Huilin Li, William Ramses Bishai, K. Heran Darwin

Research output: Contribution to journalArticle

Abstract

Ubiquitin (Ub) provides the recognition and specificity required to deliver proteins to the eukaryotic proteasome for destruction. Prokaryotic ubiquitin-like protein (Pup) is functionally analogous to Ub in Mycobacterium tuberculosis (Mtb), as it dooms proteins to the Mtb proteasome. Studies suggest that Pup and Ub do not share similar mechanisms of activation and conjugation to target proteins. Dop (deamidase of Pup; Mtb Rv2112c/MT2172) deamidates the C-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA). While studies have shed light on the conjugation of Pup to proteins, it was not known if Pup could be removed from substrates in a manner analogous to the deconjugation of Ub from eukaryotic proteins. Here, we show that Mycobacteria have a " depupylase" activity provided by Dop. The discovery of a depupylase strengthens the parallels between the Pup- and Ub-tagging systems of prokaryotes and eukaryotes, respectively.

Original languageEnglish (US)
Pages (from-to)821-827
Number of pages7
JournalMolecular Cell
Volume39
Issue number5
DOIs
StatePublished - Sep 2010

Fingerprint

Ubiquitins
Proteasome Endopeptidase Complex
Ubiquitin
Mycobacterium tuberculosis
Proteins
Mycobacterium
Eukaryota
Glutamine
Ligation
Glutamic Acid

Keywords

  • Microbio
  • Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

"Depupylation" of Prokaryotic Ubiquitin-like Protein from Mycobacterial Proteasome Substrates. / Burns, Kristin E.; Cerda-Maira, Francisca A.; Wang, Tao; Li, Huilin; Bishai, William Ramses; Darwin, K. Heran.

In: Molecular Cell, Vol. 39, No. 5, 09.2010, p. 821-827.

Research output: Contribution to journalArticle

Burns, Kristin E. ; Cerda-Maira, Francisca A. ; Wang, Tao ; Li, Huilin ; Bishai, William Ramses ; Darwin, K. Heran. / "Depupylation" of Prokaryotic Ubiquitin-like Protein from Mycobacterial Proteasome Substrates. In: Molecular Cell. 2010 ; Vol. 39, No. 5. pp. 821-827.
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