Depletion of peripheral-type benzodiazepine receptors after hypophysectomy in rat adrenal gland and testis

Robert R.H. Anholt; Errol B. De Souza; Michael J. Kuhar; Solomon H. Snyder

doi:10.1016/0014-2999(85)90026-3

Depletion of peripheral-type benzodiazepine receptors after hypophysectomy in rat adrenal gland and testis

Robert R.H. Anholt, Errol B. De Souza, Michael J. Kuhar, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

We examined the effect of hypophysectomy in rats on peripheral-type benzodiazepine receptors in the adrenal gland and testis. Following removal of the pituitary, binding sites for [³H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, are depleted in these tissues in parallel with their atrophy. This reduction in receptor levels appears to be a primary effect of the hypophysectomy, since castration has no effect on benzodiazepine receptors in the adrenal gland, nor does adrenalectomy influence testicular levels of these receptors. Our results indicate that peripheral-type benzodiazepine receptors in these steroidogenic organs are localized to cells which are dependent on trophic maintenance by the pituitary.

Original language	English (US)
Pages (from-to)	41-46
Number of pages	6
Journal	European Journal of Pharmacology
Volume	110
Issue number	1
DOIs	https://doi.org/10.1016/0014-2999(85)90026-3
State	Published - Mar 26 1985

Keywords

Endocrine glands
Hypophysectomy
Peripheral-type benzodiazepine receptors
Ro5-4864

ASJC Scopus subject areas

Pharmacology

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10.1016/0014-2999(85)90026-3

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title = "Depletion of peripheral-type benzodiazepine receptors after hypophysectomy in rat adrenal gland and testis",

abstract = "We examined the effect of hypophysectomy in rats on peripheral-type benzodiazepine receptors in the adrenal gland and testis. Following removal of the pituitary, binding sites for [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, are depleted in these tissues in parallel with their atrophy. This reduction in receptor levels appears to be a primary effect of the hypophysectomy, since castration has no effect on benzodiazepine receptors in the adrenal gland, nor does adrenalectomy influence testicular levels of these receptors. Our results indicate that peripheral-type benzodiazepine receptors in these steroidogenic organs are localized to cells which are dependent on trophic maintenance by the pituitary.",

keywords = "Endocrine glands, Hypophysectomy, Peripheral-type benzodiazepine receptors, Ro5-4864",

author = "Anholt, {Robert R.H.} and {De Souza}, {Errol B.} and Kuhar, {Michael J.} and Snyder, {Solomon H.}",

note = "Funding Information: This work was supported by a grant of International Flavors and Fragrances Incorporated. M.J.K. is a recipient of R.C.D.A. award MH-00053 and S.H.S. is a recipient of R.S.A. award DA-00074. We thank Dr. Terry R. Brown for the determination of plasma concentrations of testosterone. We thank Gregory Mack, Susan Goehring, Naomi Taylor and Roberta Proctor for excellent technical assistance and Dawn C. Dodson for secretarial assistance.",

year = "1985",

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doi = "10.1016/0014-2999(85)90026-3",

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AU - Anholt, Robert R.H.

AU - De Souza, Errol B.

AU - Kuhar, Michael J.

AU - Snyder, Solomon H.

N1 - Funding Information: This work was supported by a grant of International Flavors and Fragrances Incorporated. M.J.K. is a recipient of R.C.D.A. award MH-00053 and S.H.S. is a recipient of R.S.A. award DA-00074. We thank Dr. Terry R. Brown for the determination of plasma concentrations of testosterone. We thank Gregory Mack, Susan Goehring, Naomi Taylor and Roberta Proctor for excellent technical assistance and Dawn C. Dodson for secretarial assistance.

PY - 1985/3/26

Y1 - 1985/3/26

N2 - We examined the effect of hypophysectomy in rats on peripheral-type benzodiazepine receptors in the adrenal gland and testis. Following removal of the pituitary, binding sites for [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, are depleted in these tissues in parallel with their atrophy. This reduction in receptor levels appears to be a primary effect of the hypophysectomy, since castration has no effect on benzodiazepine receptors in the adrenal gland, nor does adrenalectomy influence testicular levels of these receptors. Our results indicate that peripheral-type benzodiazepine receptors in these steroidogenic organs are localized to cells which are dependent on trophic maintenance by the pituitary.

AB - We examined the effect of hypophysectomy in rats on peripheral-type benzodiazepine receptors in the adrenal gland and testis. Following removal of the pituitary, binding sites for [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, are depleted in these tissues in parallel with their atrophy. This reduction in receptor levels appears to be a primary effect of the hypophysectomy, since castration has no effect on benzodiazepine receptors in the adrenal gland, nor does adrenalectomy influence testicular levels of these receptors. Our results indicate that peripheral-type benzodiazepine receptors in these steroidogenic organs are localized to cells which are dependent on trophic maintenance by the pituitary.

KW - Endocrine glands

KW - Hypophysectomy

KW - Peripheral-type benzodiazepine receptors

KW - Ro5-4864

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Depletion of peripheral-type benzodiazepine receptors after hypophysectomy in rat adrenal gland and testis

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Keywords

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