TY - JOUR
T1 - Depletion of complement does not impact initiation of xenobiotic-induced autoimmune disease
AU - Cauvi, David M.
AU - Toomey, Christopher B.
AU - Pollard, K. Michael
PY - 2012/4
Y1 - 2012/4
N2 - Deficiency in Daf1, a complement regulatory protein, has been shown to exacerbate development of various autoimmune diseases and recent studies have suggested that this may be explained by Daf1 acting to limit T-cell hyper-responsiveness. It has been suggested that the absence of Daf1 aggravates autoimmune disease in a complement-dependent manner, but others have shown that activation of T cells in the absence of Daf1 can be complement independent. However, the relationship between Daf1, complement components, lymphocyte activation, cytokine expression and antibody production remains to be determined in mice that are not Daf1 deficient. We have recently demonstrated, in murine mercury-induced autoimmunity (mHgIA), that an accumulation of CD44 high Daf lowCD4 + T cells is associated with the development of autoimmunity. In this study we observed that complement depletion does not affect the accumulation of activated CD4 + T cells, elevation of splenic interleukin-4 expression and autoantibody production in mHgIA. In addition, neither the accumulation of CD44 highDaf lowCD4 + T cells nor the down-regulation of Daf1 expression on CD4 + T cells was influenced by a lack of complement. In conclusion, these studies show that initiating events in xenobiotic-induced autoimmunity, including lymphocyte activation, cytokine expression and autoantibody production, are not dependent on complement.
AB - Deficiency in Daf1, a complement regulatory protein, has been shown to exacerbate development of various autoimmune diseases and recent studies have suggested that this may be explained by Daf1 acting to limit T-cell hyper-responsiveness. It has been suggested that the absence of Daf1 aggravates autoimmune disease in a complement-dependent manner, but others have shown that activation of T cells in the absence of Daf1 can be complement independent. However, the relationship between Daf1, complement components, lymphocyte activation, cytokine expression and antibody production remains to be determined in mice that are not Daf1 deficient. We have recently demonstrated, in murine mercury-induced autoimmunity (mHgIA), that an accumulation of CD44 high Daf lowCD4 + T cells is associated with the development of autoimmunity. In this study we observed that complement depletion does not affect the accumulation of activated CD4 + T cells, elevation of splenic interleukin-4 expression and autoantibody production in mHgIA. In addition, neither the accumulation of CD44 highDaf lowCD4 + T cells nor the down-regulation of Daf1 expression on CD4 + T cells was influenced by a lack of complement. In conclusion, these studies show that initiating events in xenobiotic-induced autoimmunity, including lymphocyte activation, cytokine expression and autoantibody production, are not dependent on complement.
KW - Autoimmunity
KW - Complement
KW - Decay accelerating factor
KW - Mercury
KW - Mouse
UR - http://www.scopus.com/inward/record.url?scp=84857698712&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857698712&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2567.2011.03545.x
DO - 10.1111/j.1365-2567.2011.03545.x
M3 - Article
C2 - 22136142
AN - SCOPUS:84857698712
VL - 135
SP - 333
EP - 343
JO - Immunology
JF - Immunology
SN - 0019-2805
IS - 4
ER -