Dependence on the Lazaro Phosphatidic Acid Phosphatase for the Maximum Light Response

Young Kwon, Craig Montell

Research output: Contribution to journalArticle

Abstract

The Drosophila phototransduction cascade serves as a paradigm for characterizing the regulation of sensory signaling and TRP channels in vivo [1, 2]. Activation of these channels requires phospholipase C (PLC) and may depend on subsequent production of diacylglycerol (DAG) and downstream metabolites [3, 4]. DAG could potentially be produced through a second pathway involving the combined activities of a phospholipase D (PLD) [5] and a phosphatidic acid (PA) phosphatase (PAP). However, a role for a PAP in the regulation of TRP channels has not been described. Here, we report the identification of a PAP, referred to as Lazaro (Laza). Mutations in laza caused a reduction in the light response and faster termination kinetics. Loss of laza suppressed the severity of the phenotype caused by mutation of the DAG kinase, RDGA [6, 7], indicating that Laza functions in opposition to RDGA. We also showed that the retinal degeneration resulting from overexpression of the PLD [5] was suppressed by elimination of Laza. These data demonstrate a requirement for a PLD/PAP-dependent pathway for achieving the maximal light response. The genetic interactions with both rdgA and Pld indicate that Laza functions in the convergence of both PLC- and PLD-coupled signaling in vivo.

Original languageEnglish (US)
Pages (from-to)723-729
Number of pages7
JournalCurrent Biology
Volume16
Issue number7
DOIs
StatePublished - Apr 4 2006

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Phosphatidate Phosphatase
Phospholipase D
phospholipase D
acid phosphatase
Phosphoric Monoester Hydrolases
Light
phospholipase C
Diglycerides
Type C Phospholipases
diacylglycerols
diacylglycerol kinase
Diacylglycerol Kinase
Light Signal Transduction
phototransduction
mutation
Retinal Degeneration
Mutation
Metabolites
Drosophila
Chemical activation

Keywords

  • MOLNEURO
  • SIGNALING

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Dependence on the Lazaro Phosphatidic Acid Phosphatase for the Maximum Light Response. / Kwon, Young; Montell, Craig.

In: Current Biology, Vol. 16, No. 7, 04.04.2006, p. 723-729.

Research output: Contribution to journalArticle

Kwon, Young ; Montell, Craig. / Dependence on the Lazaro Phosphatidic Acid Phosphatase for the Maximum Light Response. In: Current Biology. 2006 ; Vol. 16, No. 7. pp. 723-729.
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