Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction

Kartik Venkatachalam, David Wasserman, Xiaoyue Wang, Ruoxia Li, Eric Mills, Rebecca Elsaesser, Hong Sheng Li, Craig Montell

Research output: Contribution to journalArticle

Abstract

Normal termination of signaling is essential to reset signaling cascades, especially those such as phototransduction that are turned on and off with great rapidity. Genetic approaches in Drosophila led to the identification of several proteins required for termination, including protein kinase C (PKC), NINAC (neither inactivation nor afterpotential C) p174, which consists of fused protein kinase and myosin domains, and a PDZ (postsynaptic density-95/Discs Large/zona occludens-1) scaffold protein, INAD (inactivation no afterpotential D). Here, we describe a mutation affecting a poorly characterized but evolutionarily conserved protein, Retinophilin (Retin), which is expressed primarily in the phototransducing compartment of photoreceptor cells, the rhabdomeres. Retin and NINAC formed a complex and were mutually dependent on each other for expression. Loss of retin resulted in an age-dependent impairment in termination of phototransduction. Mutations that affect termination of the photoresponse typically lead to a reduction in levels of the major rhodopsin (Rh1) to attenuate signaling. Consistent with the slower termination in retin1, the mutant photoreceptor cells exhibited increased endocytosis of Rh1 and a decline in Rh1 protein. The slower termination in retin1 was a consequence of a cascade of defects, which began with the reduction in NINAC p174 levels. The diminished p174 concentration caused a decrease in INAD. Because PKC requires interaction with INAD for protein stability, this leads to reduction in PKC levels. The decline in PKC was age dependent and paralleled the onset of the termination phenotype in retin 1 mutant flies. We conclude that the slower termination of the photoresponse in retin1 resulted from a requirement for the Retin/NINAC complex for stability of INAD and PKC.

Original languageEnglish (US)
Pages (from-to)11337-11345
Number of pages9
JournalJournal of Neuroscience
Volume30
Issue number34
DOIs
StatePublished - Aug 25 2010

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Light Signal Transduction
Myosins
Protein Kinase C
Photoreceptor Cells
Zonula Occludens-1 Protein
PDZ Domains
Post-Synaptic Density
Mutation
Proteins
Rhodopsin
Protein Stability
Endocytosis
Diptera
Protein Kinases
Drosophila
Phenotype

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Venkatachalam, K., Wasserman, D., Wang, X., Li, R., Mills, E., Elsaesser, R., ... Montell, C. (2010). Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction. Journal of Neuroscience, 30(34), 11337-11345. https://doi.org/10.1523/JNEUROSCI.2709-10.2010

Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction. / Venkatachalam, Kartik; Wasserman, David; Wang, Xiaoyue; Li, Ruoxia; Mills, Eric; Elsaesser, Rebecca; Li, Hong Sheng; Montell, Craig.

In: Journal of Neuroscience, Vol. 30, No. 34, 25.08.2010, p. 11337-11345.

Research output: Contribution to journalArticle

Venkatachalam, K, Wasserman, D, Wang, X, Li, R, Mills, E, Elsaesser, R, Li, HS & Montell, C 2010, 'Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction', Journal of Neuroscience, vol. 30, no. 34, pp. 11337-11345. https://doi.org/10.1523/JNEUROSCI.2709-10.2010
Venkatachalam, Kartik ; Wasserman, David ; Wang, Xiaoyue ; Li, Ruoxia ; Mills, Eric ; Elsaesser, Rebecca ; Li, Hong Sheng ; Montell, Craig. / Dependence on a retinophilin/myosin complex for stability of PKC and INAD and termination of phototransduction. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 34. pp. 11337-11345.
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