Dendritic cells respond to influenza virus through TLR7- and PKR-independent pathways

Winfried Barchet, Anne Krug, Marina Cella, Celeste Newby, Jens A.A. Fischer, Andrzej Dzionek, Andrew Pekosz, Marco Colonna

Research output: Contribution to journalArticle

Abstract

Natural interferon-producing cells (IPC) secrete type I IFN (IFN-α and -β) in response to influenza virus. This process is independent of viral replication and is mediated by Toll-like receptor 7 (TLR7), which recognizes single-stranded RNA (ssRNA). DC also express TLR7 but its function in DC response to influenza virus is unknown. To address this, we compared the DC and IPC responses to influenza virus and ssRNA oligoribonucleotides (ORN) that activate TLR7. When stimulated by ORN in vitro and in vivo, DC matured and produced inflammatory cytokines but not IFN-α. DC did secrete IFN-α in response to influenza virus. However, this response was independent of TLR7 signaling and required viral replication but not dsRNA-activated protein kinase (PKR). We conclude that DC and IPC are hard-wired to secrete IFN-α via different pathways, reflecting their complementary but distinct roles in anti-viral immunity.

Original languageEnglish (US)
Pages (from-to)236-242
Number of pages7
JournalEuropean Journal of Immunology
Volume35
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Keywords

  • Dendritic cell
  • Interferon-α
  • Viral

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Barchet, W., Krug, A., Cella, M., Newby, C., Fischer, J. A. A., Dzionek, A., Pekosz, A., & Colonna, M. (2005). Dendritic cells respond to influenza virus through TLR7- and PKR-independent pathways. European Journal of Immunology, 35(1), 236-242. https://doi.org/10.1002/eji.200425583