TY - JOUR
T1 - Dendritic cell immunization route determines integrin expression and lymphoid and nonlymphoid tissue distribution of CD8 T cells
AU - Sheasley-O'Neill, Stacey L.
AU - Brinkman, C. Colin
AU - Ferguson, Andrew R.
AU - Dispenza, Melanie C.
AU - Engelhard, Victor H.
PY - 2007/2/1
Y1 - 2007/2/1
N2 - Exogenous dendritic cells (bone marrow-derived dendritic cell (BMDC)) display restricted trafficking in vivo after injection into mice, but the route(s) by which they generate gut-homing effector cells is unclear. Mesenteric lymph nodes (LN) and spleen were differentially targeted by i.p. and i.v. administration of BMDC, respectively, whereas mediastinal LN were targeted by both routes. BMDC injected by either route activated CD8+ T cells to lip-regulate both α4β1 and α 4β7 integrals. However, the lymphoid compartment in which activation occurred determined their expression kinetics, magnitude, and population distribution. Only T cells activated in mesenteric LN after i.p. immunization expressed high levels of α4β7, which also correlated with localization to small intestine. These α4β7high cells also redistributed to mediastinal LN in a manner sensitive to treatment with α 4β7 blocking Abs, but not to mucosal addressin cell adhesion molecule-1 blocking Abs. Our results demonstrate the importance of lymphoid compartment, as dictated by immunization route, in determining integrin expression on activated T cells and their distribution in lymphoid and nonlymphoid tissues.
AB - Exogenous dendritic cells (bone marrow-derived dendritic cell (BMDC)) display restricted trafficking in vivo after injection into mice, but the route(s) by which they generate gut-homing effector cells is unclear. Mesenteric lymph nodes (LN) and spleen were differentially targeted by i.p. and i.v. administration of BMDC, respectively, whereas mediastinal LN were targeted by both routes. BMDC injected by either route activated CD8+ T cells to lip-regulate both α4β1 and α 4β7 integrals. However, the lymphoid compartment in which activation occurred determined their expression kinetics, magnitude, and population distribution. Only T cells activated in mesenteric LN after i.p. immunization expressed high levels of α4β7, which also correlated with localization to small intestine. These α4β7high cells also redistributed to mediastinal LN in a manner sensitive to treatment with α 4β7 blocking Abs, but not to mucosal addressin cell adhesion molecule-1 blocking Abs. Our results demonstrate the importance of lymphoid compartment, as dictated by immunization route, in determining integrin expression on activated T cells and their distribution in lymphoid and nonlymphoid tissues.
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U2 - 10.4049/jimmunol.178.3.1512
DO - 10.4049/jimmunol.178.3.1512
M3 - Article
C2 - 17237400
AN - SCOPUS:33846504106
SN - 0022-1767
VL - 178
SP - 1512
EP - 1522
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -