Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress

Niral Gandhi, Zhitao Su, Xiaobo Zhang, Eugene A. Volpe, Flavia S.A. Pelegrino, Salman A. Rahman, De Quan Li, Stephen C. Pflugfelder, Cintia S. de Paiva

Research output: Contribution to journalArticle

Abstract

TSP-1 is a physiologic activator of TGF-β, a critical induction factor for Th17-mediated immunity. The purpose of this study was to investigate the role of TSP-1 in the induction of the Th17 ocular surface response to DS. TSP-1KO and WT mice were subjected to DS5 and DS10), and parameters of ocular surface disease, including corneal barrier function, conjunctival CD4+ T cell infiltration, and GC density, were evaluated. TSP1KO mice subjected to DS had less corneal barrier disruption, reduced loss of PAS+ GC, and decreased CD4+ T cell infiltration in the conjunctiva. In contrast to WT, TSP-1KO mice failed to up-regulate MMP-3 and MMP-9 mRNA transcripts in the cornea and IL-17A mRNA transcripts in the conjunctiva. RAG-1KO recipients of adoptively transferred CD4+ T cells isolated from TSP-1KO mice subjected to DS5 showed milder dry-eye phenotype and less conjunctival inflammation than recipients of CD4+ T cells from DS5 WT control. Reconstitution of TSP-1KO mice with WT DCs prior to DS reversed the resistance of the TSP-1KO to DS-induced immunopathology. In conclusion, DC-derived TSP-1 is critical for generating the Th17 ocular surface response to DS.

Original languageEnglish (US)
Pages (from-to)1293-1301
Number of pages9
JournalJournal of Leukocyte Biology
Volume94
Issue number6
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

Fingerprint

Thrombospondin 1
Dendritic Cells
T-Lymphocytes
Conjunctiva
Matrix Metalloproteinases
Messenger RNA
Interleukin-17
Eye Diseases
Cornea
Immunity
Up-Regulation
Inflammation
Phenotype

Keywords

  • Conjunctiva
  • Corneal permeability
  • Dry
  • Eye
  • Goblet cells
  • IFN-γ
  • Il-17A
  • TGF-β

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Gandhi, N., Su, Z., Zhang, X., Volpe, E. A., Pelegrino, F. S. A., Rahman, S. A., ... de Paiva, C. S. (2013). Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress. Journal of Leukocyte Biology, 94(6), 1293-1301. https://doi.org/10.1189/jlb.1012524

Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress. / Gandhi, Niral; Su, Zhitao; Zhang, Xiaobo; Volpe, Eugene A.; Pelegrino, Flavia S.A.; Rahman, Salman A.; Li, De Quan; Pflugfelder, Stephen C.; de Paiva, Cintia S.

In: Journal of Leukocyte Biology, Vol. 94, No. 6, 01.12.2013, p. 1293-1301.

Research output: Contribution to journalArticle

Gandhi, N, Su, Z, Zhang, X, Volpe, EA, Pelegrino, FSA, Rahman, SA, Li, DQ, Pflugfelder, SC & de Paiva, CS 2013, 'Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress', Journal of Leukocyte Biology, vol. 94, no. 6, pp. 1293-1301. https://doi.org/10.1189/jlb.1012524
Gandhi, Niral ; Su, Zhitao ; Zhang, Xiaobo ; Volpe, Eugene A. ; Pelegrino, Flavia S.A. ; Rahman, Salman A. ; Li, De Quan ; Pflugfelder, Stephen C. ; de Paiva, Cintia S. / Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress. In: Journal of Leukocyte Biology. 2013 ; Vol. 94, No. 6. pp. 1293-1301.
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abstract = "TSP-1 is a physiologic activator of TGF-β, a critical induction factor for Th17-mediated immunity. The purpose of this study was to investigate the role of TSP-1 in the induction of the Th17 ocular surface response to DS. TSP-1KO and WT mice were subjected to DS5 and DS10), and parameters of ocular surface disease, including corneal barrier function, conjunctival CD4+ T cell infiltration, and GC density, were evaluated. TSP1KO mice subjected to DS had less corneal barrier disruption, reduced loss of PAS+ GC, and decreased CD4+ T cell infiltration in the conjunctiva. In contrast to WT, TSP-1KO mice failed to up-regulate MMP-3 and MMP-9 mRNA transcripts in the cornea and IL-17A mRNA transcripts in the conjunctiva. RAG-1KO recipients of adoptively transferred CD4+ T cells isolated from TSP-1KO mice subjected to DS5 showed milder dry-eye phenotype and less conjunctival inflammation than recipients of CD4+ T cells from DS5 WT control. Reconstitution of TSP-1KO mice with WT DCs prior to DS reversed the resistance of the TSP-1KO to DS-induced immunopathology. In conclusion, DC-derived TSP-1 is critical for generating the Th17 ocular surface response to DS.",
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