Demonstration of retrotransposition of the Tf1 element in fission yeast

Henry L. Levin, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

Tf1, a retrotransposon from fission yeast, has LTRs and coding sequences resembling the protease, reverse transcriptase and integrase domains of retroviral pol genes. A unique aspect of Tf1 is that it contains a single open reading frame whereas other retroviruses and retrotransposons usually possess two or more open reading frames. To determine whether Tf1 can transpose, we overproduced Tf1 transcripts encoded by a plasmid copy of the element marked with a neo gene. Approximately 0.1-4.0% of the cell population acquired chromosomally inherited resistance to G418. DNA blot analysis demonstrated that such strains had acquired both Tf1 and neo specific sequences within a restriction fragment of the same size; the size of this restriction fragment varied between different isolates. Structural analysis of the cloned DNA flanking the Tf1 - neo element of two transposition candidates with the same regions in the parent strain showed that the ability to grow on G418 was due to transposition of Tf1-neo and not other types of recombination events.

Original languageEnglish (US)
Pages (from-to)1145-1153
Number of pages9
JournalThe EMBO journal
Volume11
Issue number3
StatePublished - 1992

Fingerprint

Retroelements
Schizosaccharomyces
Yeast
Open Reading Frames
Demonstrations
Genes
pol Genes
Integrases
RNA-Directed DNA Polymerase
DNA
Retroviridae
Structural analysis
Genetic Recombination
Plasmids
Peptide Hydrolases
Cells
Population
antibiotic G 418

Keywords

  • Fbp promoter
  • Retrotransposition
  • Retrotransposon
  • Schizosaccharomcyes pombe
  • Tf1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Levin, H. L., & Boeke, J. D. (1992). Demonstration of retrotransposition of the Tf1 element in fission yeast. The EMBO journal, 11(3), 1145-1153.

Demonstration of retrotransposition of the Tf1 element in fission yeast. / Levin, Henry L.; Boeke, Jef D.

In: The EMBO journal, Vol. 11, No. 3, 1992, p. 1145-1153.

Research output: Contribution to journalArticle

Levin, HL & Boeke, JD 1992, 'Demonstration of retrotransposition of the Tf1 element in fission yeast', The EMBO journal, vol. 11, no. 3, pp. 1145-1153.
Levin, Henry L. ; Boeke, Jef D. / Demonstration of retrotransposition of the Tf1 element in fission yeast. In: The EMBO journal. 1992 ; Vol. 11, No. 3. pp. 1145-1153.
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