Demonstration of retrotransposition of the Tf1 element in fission yeast

H. L. Levin, J. D. Boeke

Research output: Contribution to journalArticle

Abstract

Tf1, a retrotransposon from fission yeast, has LTRs and coding sequences resembling the protease, reverse transcriptase and integrase domains of retroviral pol genes. A unique aspect of Tf1 is that it contains a single open reading frame whereas other retroviruses and retrotransposons usually possess two or more open reading frames. To determine whether Tf1 can transpose, we overproduced Tf1 transcripts encoded by a plasmid copy of the element marked with a neo gene. Approximately 0.1-4.0% of the cell population acquired chromosomally inherited resistance to G418. DNA blot analysis demonstrated that such strains had acquired both Tf1 and neo specific sequences within a restriction fragment of the same size; the size of this restriction fragment varied between different isolates. Structural analysis of the cloned DNA flanking the Tf1-neo element of two transposition candidates with the same regions in the parent strain showed that the ability to grow on G418 was due to transposition of Tf1-neo and not other types of recombination events.

Original languageEnglish (US)
Pages (from-to)1145-1153
Number of pages9
JournalEMBO Journal
Volume11
Issue number3
DOIs
StatePublished - 1992

Keywords

  • Retrotransposition
  • Retrotransposon
  • Schizosaccharomyces pombe
  • Tf1
  • fbp promoter

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Demonstration of retrotransposition of the Tf1 element in fission yeast'. Together they form a unique fingerprint.

Cite this