Demonstration of Inhibition of Mediator Release From Human Mast Cells by Azatadine Base: In Vivo and In Vitro Evaluation

Alkis G. Togias, Robert M. Naclerio, Jane Warner, David Proud, Anne Kagey-Sobotka, Indira Nimmagadda, Philip S. Norman, Lawrence M. Lichtenstein

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro experimentation using dispersed human lung mast cells demonstrated that azatadine base, a compound with known H1-antihistamine properties, inhibited anti-IgE-induced release of histamine and leukotriene C4 by 45% and 85%, respectively. To assess the clinical relevance of these findings and to compare in vitro mast cell data with results obtained in vivo, nasally instilled azatadine was tested in a double-blind, placebo-controlled clinical trial in which nasal challenges with antigen were performed on eight allergic individuals. Pretreatment with azatadine significantly suppressed the number of sneezes following antigen challenge and inhibited the associated elevations in histamine, kinins, and enzyme(s) hydrolyzing the artificial substrate N-α-tosyl-L-arginine-methyl-ester in nasal secretions, whereas placebo was inactive. Hence, we showed agreement between our in vitro and in vivo experimental models of the allergic reaction. Topical application of azatadine base has the potential to become an effective antiallergic treatment.

Original languageEnglish (US)
Pages (from-to)225-229
Number of pages5
JournalJAMA: The Journal of the American Medical Association
Volume255
Issue number2
DOIs
StatePublished - Jan 10 1986

ASJC Scopus subject areas

  • Medicine(all)

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