DEMONSTRATION OF CIRCULATING 1,24,25‐TRIHYDROXYVITAMIN D3 IN MAN BY RADIOIMMUNOASSAY

T. L. CLEMENS; L. J. FRAHER; L. M. SANDLER; J. L.H. O'RIORDAN

doi:10.1111/j.1365-2265.1982.tb00725.x

DEMONSTRATION OF CIRCULATING 1,24,25‐TRIHYDROXYVITAMIN D₃ IN MAN BY RADIOIMMUNOASSAY

T. L. CLEMENS, L. J. FRAHER, L. M. SANDLER, J. L.H. O'RIORDAN

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

1,24,25‐trihydroxyvitamin D₃ has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25‐trihydroxyvitamin D₃ was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH)₃D₃ in normal subjects ranged from 9·3 to 18·5 pmol/l but was not detectable (> 2·3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D₃.

Original language	English (US)
Pages (from-to)	337-343
Number of pages	7
Journal	Clinical Endocrinology
Volume	16
Issue number	4
DOIs	https://doi.org/10.1111/j.1365-2265.1982.tb00725.x
State	Published - Apr 1982
Externally published	Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1111/j.1365-2265.1982.tb00725.x

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title = "DEMONSTRATION OF CIRCULATING 1,24,25‐TRIHYDROXYVITAMIN D3 IN MAN BY RADIOIMMUNOASSAY",

abstract = "1,24,25‐trihydroxyvitamin D3 has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25‐trihydroxyvitamin D3 was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH)3D3 in normal subjects ranged from 9·3 to 18·5 pmol/l but was not detectable (> 2·3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D3.",

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AU - CLEMENS, T. L.

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AU - SANDLER, L. M.

AU - O'RIORDAN, J. L.H.

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AB - 1,24,25‐trihydroxyvitamin D3 has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25‐trihydroxyvitamin D3 was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH)3D3 in normal subjects ranged from 9·3 to 18·5 pmol/l but was not detectable (> 2·3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D3.

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DEMONSTRATION OF CIRCULATING 1,24,25‐TRIHYDROXYVITAMIN D₃ IN MAN BY RADIOIMMUNOASSAY

Abstract

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