DEMONSTRATION OF CIRCULATING 1,24,25‐TRIHYDROXYVITAMIN D3 IN MAN BY RADIOIMMUNOASSAY

T. L. CLEMENS, L. J. FRAHER, L. M. SANDLER, J. L.H. O'RIORDAN

Research output: Contribution to journalArticlepeer-review

Abstract

1,24,25‐trihydroxyvitamin D3 has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25‐trihydroxyvitamin D3 was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH)3D3 in normal subjects ranged from 9·3 to 18·5 pmol/l but was not detectable (> 2·3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D3.

Original languageEnglish (US)
Pages (from-to)337-343
Number of pages7
JournalClinical Endocrinology
Volume16
Issue number4
DOIs
StatePublished - Apr 1982
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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