Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis

G. P. Melcher, K. D. Reed, M. G. Rinaldi, J. W. Lee, P. A. Pizzo, T. J. Walsh

Research output: Contribution to journalArticle

Abstract

Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.

Original languageEnglish (US)
Pages (from-to)192-196
Number of pages5
JournalJournal of Clinical Microbiology
Volume29
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Trichosporonosis
Cell Wall
Antigens
Trichosporon
Gold Colloid
Cryptococcus neoformans
Immunoelectron Microscopy
Antibodies
Goats
Capsules
Polysaccharides
Theoretical Models
Immunoglobulin G
Monoclonal Antibodies
Rabbits
Lung
Infection
cryptococcal polysaccharide

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Melcher, G. P., Reed, K. D., Rinaldi, M. G., Lee, J. W., Pizzo, P. A., & Walsh, T. J. (1991). Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis. Journal of Clinical Microbiology, 29(1), 192-196.

Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis. / Melcher, G. P.; Reed, K. D.; Rinaldi, M. G.; Lee, J. W.; Pizzo, P. A.; Walsh, T. J.

In: Journal of Clinical Microbiology, Vol. 29, No. 1, 1991, p. 192-196.

Research output: Contribution to journalArticle

Melcher, G. P. ; Reed, K. D. ; Rinaldi, M. G. ; Lee, J. W. ; Pizzo, P. A. ; Walsh, T. J. / Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis. In: Journal of Clinical Microbiology. 1991 ; Vol. 29, No. 1. pp. 192-196.
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