Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis

G. P. Melcher; K. D. Reed; M. G. Rinaldi; J. W. Lee; P. A. Pizzo; T. J. Walsh

doi:10.1128/jcm.29.1.192-196.1991

Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis

G. P. Melcher, K. D. Reed, M. G. Rinaldi, J. W. Lee, P. A. Pizzo, T. J. Walsh

Research output: Contribution to journal › Article › peer-review

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Abstract

Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.

Original language	English (US)
Pages (from-to)	192-196
Number of pages	5
Journal	Journal of clinical microbiology
Volume	29
Issue number	1
DOIs	https://doi.org/10.1128/jcm.29.1.192-196.1991
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.29.1.192-196.1991

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title = "Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis",

abstract = "Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.",

author = "Melcher, {G. P.} and Reed, {K. D.} and Rinaldi, {M. G.} and Lee, {J. W.} and Pizzo, {P. A.} and Walsh, {T. J.}",

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doi = "10.1128/jcm.29.1.192-196.1991",

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AU - Melcher, G. P.

AU - Reed, K. D.

AU - Rinaldi, M. G.

AU - Lee, J. W.

AU - Pizzo, P. A.

AU - Walsh, T. J.

PY - 1991

Y1 - 1991

N2 - Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.

AB - Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.

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Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis

Abstract

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