Delivery of plasmid DNA expression vector for keratinocyte growth factor-1 using electroporation to improve cutaneous wound healing in a septic rat model

Michael P. Lin, Guy P. Marti, Rami Dieb, Jiaai Wang, Mark Ferguson, Rabia Qaiser, Pramod Bonde, Mark D Duncan, John Harmon

Research output: Contribution to journalArticle

Abstract

We have previously shown that wound healing was improved in a diabetic mouse model of impaired wound healing following transfection with keratinocyte growth factor-1 (KGF-1) cDNA. We now extend these findings to the characterization of the effects of DNA plasmid vectors delivered to rats using electroporation (EP) in vivo in a sepsis-based model of impaired wound healing. To assess plasmid transfection and wound healing, gWIZ luciferase and PCDNA3.1/KGF-1 expression vectors were used, respectively. Cutaneous wounds were produced using an 8 mm-punch biopsy in Sprague-Dawley rats in which healing was impaired by cecal ligation-induced sepsis. We used National Institutes of Health image analysis software and histologic assessment to analyze wound closure and found that EP increased expression of gWIZ luciferase vector up to 53-fold compared with transfection without EP (p

Original languageEnglish (US)
Pages (from-to)618-624
Number of pages7
JournalWound Repair and Regeneration
Volume14
Issue number5
DOIs
StatePublished - Sep 2006

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Fibroblast Growth Factor 7
Electroporation
Wound Healing
Plasmids
Transfection
Skin
DNA
Luciferases
Sepsis
Wounds and Injuries
National Institutes of Health (U.S.)
Ligation
Sprague Dawley Rats
Software
Complementary DNA
Biopsy

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Delivery of plasmid DNA expression vector for keratinocyte growth factor-1 using electroporation to improve cutaneous wound healing in a septic rat model. / Lin, Michael P.; Marti, Guy P.; Dieb, Rami; Wang, Jiaai; Ferguson, Mark; Qaiser, Rabia; Bonde, Pramod; Duncan, Mark D; Harmon, John.

In: Wound Repair and Regeneration, Vol. 14, No. 5, 09.2006, p. 618-624.

Research output: Contribution to journalArticle

Lin, Michael P. ; Marti, Guy P. ; Dieb, Rami ; Wang, Jiaai ; Ferguson, Mark ; Qaiser, Rabia ; Bonde, Pramod ; Duncan, Mark D ; Harmon, John. / Delivery of plasmid DNA expression vector for keratinocyte growth factor-1 using electroporation to improve cutaneous wound healing in a septic rat model. In: Wound Repair and Regeneration. 2006 ; Vol. 14, No. 5. pp. 618-624.
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