Delivery of a viral antigen to the class i processing and presentation pathway by listeria monocytogenes

Georgios Ikonomidis, Yvonne Paterson, Ferdynand J. Kos, Daniel A. Portnoy

Research output: Contribution to journalArticlepeer-review

Abstract

Listeria monocytogenes is a facultative intracellular pathogen that grows in the cytoplasm of infected host cells. We examined the capacity of L. monocytogenes to introduce influenza nucleoprotein (NP) into the class I pathway of antigen presentation both in vitro and in vivo. Recombinant L. monocTtogenes secreting a fusion of listeriolysin O and NP (LLO-NP) targeted infected cells for lysis by NP-specific class I-restricted cytotoxic T cells. Antigen presentation occurred in the context of three different class I haplotypes in vitro. A hemolysin-negative L. monocytogenes strain expressing LLO-NP was able to present in a class II-restricted manner. However, it failed to target infected cells for lysis by CD8+T cells, indicating that hemolysin-dependent bacterial escape from the vacuole is necessary for class I presentation in vitro. Immunization of mice with a recombinant L. monocytogenes strain that stably expressed and secreted LLO-NP induced NPspecific CD8+cytotoxic T lymphocytes. These studies have implications for the use of L. monocytogenes to deliver potentially any antigen to the class I pathway in vivo.

Original languageEnglish (US)
Pages (from-to)2209-2218
Number of pages10
JournalJournal of Experimental Medicine
Volume180
Issue number6
DOIs
StatePublished - Dec 1 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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