Delivery mode and the transition of pioneering gut-microbiota structure, composition and predicted metabolic function

Noel Mueller, Hakdong Shin, Aline Pizoni, Isabel C. Werlang, Ursula Matte, Marcelo Z. Goldani, Helena A.S. Goldani, Maria G. Dominguez-Bello

Research output: Contribution to journalArticle

Abstract

Cesarean (C-section) delivery, recently shown to cause excess weight gain in mice, perturbs human neonatal gut microbiota development due to the lack of natural mother-to-newborn transfer of microbes. Neonates excrete first the in-utero intestinal content (referred to as meconium) hours after birth, followed by intestinal contents reflective of extra-uterine exposure (referred to as transition stool) 2 to 3 days after birth. It is not clear when the effect of C-section on the neonatal gut microbiota emerges. We examined bacterial DNA in carefully-collected meconium, and the subsequent transitional stool, from 59 neonates [13 born by scheduled C-section and 46 born by vaginal delivery] in a private hospital in Brazil. Bacterial DNA was extracted, and the V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq (San Diego, CA, USA) platform. We found evidence of bacterial DNA in the majority of meconium samples in our study. The bacterial DNA structure (i.e., beta diversity) of meconium differed significantly from that of the transitional stool microbiota. There was a significant reduction in bacterial alpha diversity (e.g., number of observed bacterial species) and change in bacterial composition (e.g., reduced Proteobacteria) in the transition from meconium to stool. However, changes in predicted microbiota metabolic function from meconium to transitional stool were only observed in vaginally-delivered neonates. Within sample comparisons showed that delivery mode was significantly associated with bacterial structure, composition and predicted microbiota metabolic function in transitional-stool samples, but not in meconium samples. Specifically, compared to vaginally delivered neonates, the transitional stool of C-section delivered neonates had lower proportions of the genera Bacteroides, Parabacteroides and Clostridium. These differences led to C-section neonates having lower predicted abundance of microbial genes related to metabolism of amino and nucleotide sugars, and higher abundance of genes related to fatty-acid metabolism, amino-acid degradation and xenobiotics biodegradation. In summary, microbiota diversity was reduced in the transition from meconium to stool, and the association of delivery mode with microbiota structure, composition and predicted metabolic unction was not observed until the passing of the transitional stool after meconium.

Original languageEnglish (US)
Article number364
JournalGenes
Volume8
Issue number12
DOIs
StatePublished - Dec 1 2017

Fingerprint

Meconium
Microbiota
Bacterial DNA
Bacterial Structures
Gastrointestinal Contents
Microbial Genes
Parturition
Amino Sugars
Gastrointestinal Microbiome
Proteobacteria
Private Hospitals
Bacteroides
Clostridium
Xenobiotics
rRNA Genes
Cesarean Section
Weight Gain
Brazil
Fatty Acids
Nucleotides

Keywords

  • Cesarean section
  • Microbial community
  • Microbiome
  • Microbiota
  • Obesity

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Mueller, N., Shin, H., Pizoni, A., Werlang, I. C., Matte, U., Goldani, M. Z., ... Dominguez-Bello, M. G. (2017). Delivery mode and the transition of pioneering gut-microbiota structure, composition and predicted metabolic function. Genes, 8(12), [364]. https://doi.org/10.3390/genes8120364

Delivery mode and the transition of pioneering gut-microbiota structure, composition and predicted metabolic function. / Mueller, Noel; Shin, Hakdong; Pizoni, Aline; Werlang, Isabel C.; Matte, Ursula; Goldani, Marcelo Z.; Goldani, Helena A.S.; Dominguez-Bello, Maria G.

In: Genes, Vol. 8, No. 12, 364, 01.12.2017.

Research output: Contribution to journalArticle

Mueller, N, Shin, H, Pizoni, A, Werlang, IC, Matte, U, Goldani, MZ, Goldani, HAS & Dominguez-Bello, MG 2017, 'Delivery mode and the transition of pioneering gut-microbiota structure, composition and predicted metabolic function', Genes, vol. 8, no. 12, 364. https://doi.org/10.3390/genes8120364
Mueller, Noel ; Shin, Hakdong ; Pizoni, Aline ; Werlang, Isabel C. ; Matte, Ursula ; Goldani, Marcelo Z. ; Goldani, Helena A.S. ; Dominguez-Bello, Maria G. / Delivery mode and the transition of pioneering gut-microbiota structure, composition and predicted metabolic function. In: Genes. 2017 ; Vol. 8, No. 12.
@article{65f8573cbe904e029ad2a4b84e9d6695,
title = "Delivery mode and the transition of pioneering gut-microbiota structure, composition and predicted metabolic function",
abstract = "Cesarean (C-section) delivery, recently shown to cause excess weight gain in mice, perturbs human neonatal gut microbiota development due to the lack of natural mother-to-newborn transfer of microbes. Neonates excrete first the in-utero intestinal content (referred to as meconium) hours after birth, followed by intestinal contents reflective of extra-uterine exposure (referred to as transition stool) 2 to 3 days after birth. It is not clear when the effect of C-section on the neonatal gut microbiota emerges. We examined bacterial DNA in carefully-collected meconium, and the subsequent transitional stool, from 59 neonates [13 born by scheduled C-section and 46 born by vaginal delivery] in a private hospital in Brazil. Bacterial DNA was extracted, and the V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq (San Diego, CA, USA) platform. We found evidence of bacterial DNA in the majority of meconium samples in our study. The bacterial DNA structure (i.e., beta diversity) of meconium differed significantly from that of the transitional stool microbiota. There was a significant reduction in bacterial alpha diversity (e.g., number of observed bacterial species) and change in bacterial composition (e.g., reduced Proteobacteria) in the transition from meconium to stool. However, changes in predicted microbiota metabolic function from meconium to transitional stool were only observed in vaginally-delivered neonates. Within sample comparisons showed that delivery mode was significantly associated with bacterial structure, composition and predicted microbiota metabolic function in transitional-stool samples, but not in meconium samples. Specifically, compared to vaginally delivered neonates, the transitional stool of C-section delivered neonates had lower proportions of the genera Bacteroides, Parabacteroides and Clostridium. These differences led to C-section neonates having lower predicted abundance of microbial genes related to metabolism of amino and nucleotide sugars, and higher abundance of genes related to fatty-acid metabolism, amino-acid degradation and xenobiotics biodegradation. In summary, microbiota diversity was reduced in the transition from meconium to stool, and the association of delivery mode with microbiota structure, composition and predicted metabolic unction was not observed until the passing of the transitional stool after meconium.",
keywords = "Cesarean section, Microbial community, Microbiome, Microbiota, Obesity",
author = "Noel Mueller and Hakdong Shin and Aline Pizoni and Werlang, {Isabel C.} and Ursula Matte and Goldani, {Marcelo Z.} and Goldani, {Helena A.S.} and Dominguez-Bello, {Maria G.}",
year = "2017",
month = "12",
day = "1",
doi = "10.3390/genes8120364",
language = "English (US)",
volume = "8",
journal = "Genes",
issn = "2073-4425",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",

}

TY - JOUR

T1 - Delivery mode and the transition of pioneering gut-microbiota structure, composition and predicted metabolic function

AU - Mueller, Noel

AU - Shin, Hakdong

AU - Pizoni, Aline

AU - Werlang, Isabel C.

AU - Matte, Ursula

AU - Goldani, Marcelo Z.

AU - Goldani, Helena A.S.

AU - Dominguez-Bello, Maria G.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Cesarean (C-section) delivery, recently shown to cause excess weight gain in mice, perturbs human neonatal gut microbiota development due to the lack of natural mother-to-newborn transfer of microbes. Neonates excrete first the in-utero intestinal content (referred to as meconium) hours after birth, followed by intestinal contents reflective of extra-uterine exposure (referred to as transition stool) 2 to 3 days after birth. It is not clear when the effect of C-section on the neonatal gut microbiota emerges. We examined bacterial DNA in carefully-collected meconium, and the subsequent transitional stool, from 59 neonates [13 born by scheduled C-section and 46 born by vaginal delivery] in a private hospital in Brazil. Bacterial DNA was extracted, and the V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq (San Diego, CA, USA) platform. We found evidence of bacterial DNA in the majority of meconium samples in our study. The bacterial DNA structure (i.e., beta diversity) of meconium differed significantly from that of the transitional stool microbiota. There was a significant reduction in bacterial alpha diversity (e.g., number of observed bacterial species) and change in bacterial composition (e.g., reduced Proteobacteria) in the transition from meconium to stool. However, changes in predicted microbiota metabolic function from meconium to transitional stool were only observed in vaginally-delivered neonates. Within sample comparisons showed that delivery mode was significantly associated with bacterial structure, composition and predicted microbiota metabolic function in transitional-stool samples, but not in meconium samples. Specifically, compared to vaginally delivered neonates, the transitional stool of C-section delivered neonates had lower proportions of the genera Bacteroides, Parabacteroides and Clostridium. These differences led to C-section neonates having lower predicted abundance of microbial genes related to metabolism of amino and nucleotide sugars, and higher abundance of genes related to fatty-acid metabolism, amino-acid degradation and xenobiotics biodegradation. In summary, microbiota diversity was reduced in the transition from meconium to stool, and the association of delivery mode with microbiota structure, composition and predicted metabolic unction was not observed until the passing of the transitional stool after meconium.

AB - Cesarean (C-section) delivery, recently shown to cause excess weight gain in mice, perturbs human neonatal gut microbiota development due to the lack of natural mother-to-newborn transfer of microbes. Neonates excrete first the in-utero intestinal content (referred to as meconium) hours after birth, followed by intestinal contents reflective of extra-uterine exposure (referred to as transition stool) 2 to 3 days after birth. It is not clear when the effect of C-section on the neonatal gut microbiota emerges. We examined bacterial DNA in carefully-collected meconium, and the subsequent transitional stool, from 59 neonates [13 born by scheduled C-section and 46 born by vaginal delivery] in a private hospital in Brazil. Bacterial DNA was extracted, and the V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq (San Diego, CA, USA) platform. We found evidence of bacterial DNA in the majority of meconium samples in our study. The bacterial DNA structure (i.e., beta diversity) of meconium differed significantly from that of the transitional stool microbiota. There was a significant reduction in bacterial alpha diversity (e.g., number of observed bacterial species) and change in bacterial composition (e.g., reduced Proteobacteria) in the transition from meconium to stool. However, changes in predicted microbiota metabolic function from meconium to transitional stool were only observed in vaginally-delivered neonates. Within sample comparisons showed that delivery mode was significantly associated with bacterial structure, composition and predicted microbiota metabolic function in transitional-stool samples, but not in meconium samples. Specifically, compared to vaginally delivered neonates, the transitional stool of C-section delivered neonates had lower proportions of the genera Bacteroides, Parabacteroides and Clostridium. These differences led to C-section neonates having lower predicted abundance of microbial genes related to metabolism of amino and nucleotide sugars, and higher abundance of genes related to fatty-acid metabolism, amino-acid degradation and xenobiotics biodegradation. In summary, microbiota diversity was reduced in the transition from meconium to stool, and the association of delivery mode with microbiota structure, composition and predicted metabolic unction was not observed until the passing of the transitional stool after meconium.

KW - Cesarean section

KW - Microbial community

KW - Microbiome

KW - Microbiota

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=85037369925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037369925&partnerID=8YFLogxK

U2 - 10.3390/genes8120364

DO - 10.3390/genes8120364

M3 - Article

VL - 8

JO - Genes

JF - Genes

SN - 2073-4425

IS - 12

M1 - 364

ER -