Abstract
Reactive oxygen species (ROS) are proposed to play a major role in telomere length alterations during aging. The mechanisms by which ROS disrupt telomeres remain unclear. In Saccharomyces cerevisiae, telomere DNA consists of TG(1-3) repeats, which are maintained primarily by telomerase. Telomere length maintenance can be modulated by the expression level of telomerase subunits and telomerase activity. Additionally, telomerase-mediated telomere repeat addition is negatively modulated by the levels of telomere-bound Rap1-Rif1-Rif2 protein complex. Using a yeast strain defective in the major peroxiredoxin Tsa1 that is involved in ROS neutralization, we have investigated the effect of defective ROS detoxification on telomere DNA, telomerase, telomere-binding proteins, and telomere length. Surprisingly, the tsa1 mutant does not show significant increase in steady-state levels of oxidative DNA lesions at telomeres. The tsa1 mutant displays abnormal telomere lengthening, and reduction in oxidative exposure alleviates this phenotype. The telomere lengthening in the tsa1 cells was abolished by disruption of Est2, subtelomeric DNA, Rap1 C-terminus, or Rif2, but not by Rif1 deletion. Although telomerase expression and activity are not altered, telomere-bound Est2 is increased, while telomere-bound Rap1 is reduced in the tsa1 mutant. We propose that defective ROS scavenging can interfere with pathways that are critical in controlling telomere length homeostasis. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 635-644 |
| Number of pages | 10 |
| Journal | Aging Cell |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2013 |
| Externally published | Yes |
Fingerprint
Keywords
- Peroxiredoxin
- Reactive oxygen species
- Telomerase
- Telomere length
ASJC Scopus subject areas
- Aging
- Cell Biology
Cite this
Deletion of the major peroxiredoxin Tsa1 alters telomere length homeostasis. / Lu, Jian; Vallabhaneni, Haritha; Yin, Jinhu; Liu, Yie.
In: Aging Cell, Vol. 12, No. 4, 08.2013, p. 635-644.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Deletion of the major peroxiredoxin Tsa1 alters telomere length homeostasis
AU - Lu, Jian
AU - Vallabhaneni, Haritha
AU - Yin, Jinhu
AU - Liu, Yie
PY - 2013/8
Y1 - 2013/8
N2 - Reactive oxygen species (ROS) are proposed to play a major role in telomere length alterations during aging. The mechanisms by which ROS disrupt telomeres remain unclear. In Saccharomyces cerevisiae, telomere DNA consists of TG(1-3) repeats, which are maintained primarily by telomerase. Telomere length maintenance can be modulated by the expression level of telomerase subunits and telomerase activity. Additionally, telomerase-mediated telomere repeat addition is negatively modulated by the levels of telomere-bound Rap1-Rif1-Rif2 protein complex. Using a yeast strain defective in the major peroxiredoxin Tsa1 that is involved in ROS neutralization, we have investigated the effect of defective ROS detoxification on telomere DNA, telomerase, telomere-binding proteins, and telomere length. Surprisingly, the tsa1 mutant does not show significant increase in steady-state levels of oxidative DNA lesions at telomeres. The tsa1 mutant displays abnormal telomere lengthening, and reduction in oxidative exposure alleviates this phenotype. The telomere lengthening in the tsa1 cells was abolished by disruption of Est2, subtelomeric DNA, Rap1 C-terminus, or Rif2, but not by Rif1 deletion. Although telomerase expression and activity are not altered, telomere-bound Est2 is increased, while telomere-bound Rap1 is reduced in the tsa1 mutant. We propose that defective ROS scavenging can interfere with pathways that are critical in controlling telomere length homeostasis. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.
AB - Reactive oxygen species (ROS) are proposed to play a major role in telomere length alterations during aging. The mechanisms by which ROS disrupt telomeres remain unclear. In Saccharomyces cerevisiae, telomere DNA consists of TG(1-3) repeats, which are maintained primarily by telomerase. Telomere length maintenance can be modulated by the expression level of telomerase subunits and telomerase activity. Additionally, telomerase-mediated telomere repeat addition is negatively modulated by the levels of telomere-bound Rap1-Rif1-Rif2 protein complex. Using a yeast strain defective in the major peroxiredoxin Tsa1 that is involved in ROS neutralization, we have investigated the effect of defective ROS detoxification on telomere DNA, telomerase, telomere-binding proteins, and telomere length. Surprisingly, the tsa1 mutant does not show significant increase in steady-state levels of oxidative DNA lesions at telomeres. The tsa1 mutant displays abnormal telomere lengthening, and reduction in oxidative exposure alleviates this phenotype. The telomere lengthening in the tsa1 cells was abolished by disruption of Est2, subtelomeric DNA, Rap1 C-terminus, or Rif2, but not by Rif1 deletion. Although telomerase expression and activity are not altered, telomere-bound Est2 is increased, while telomere-bound Rap1 is reduced in the tsa1 mutant. We propose that defective ROS scavenging can interfere with pathways that are critical in controlling telomere length homeostasis. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.
KW - Peroxiredoxin
KW - Reactive oxygen species
KW - Telomerase
KW - Telomere length
UR - http://www.scopus.com/inward/record.url?scp=84880506675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880506675&partnerID=8YFLogxK
U2 - 10.1111/acel.12085
DO - 10.1111/acel.12085
M3 - Article
C2 - 23590194
AN - SCOPUS:84880506675
VL - 12
SP - 635
EP - 644
JO - Aging Cell
JF - Aging Cell
SN - 1474-9718
IS - 4
ER -