Delayed sodium thiosulphate administration reduces Cisplatin efficacy on mouse EMT6 tumour cells in Vitro

May Sann Yee, Brian W. Blakley, Asher Begleiter, Marsha Leith

Research output: Contribution to journalArticle

Abstract

Objective: To determine whether simultaneous and/or delayed administration of sodium thiosulphate (STS) affects the oncologic effect of cisplatin or cisdiaminedichloroplatinum (CDDP) in EMT6 tumour cells in vitro. Setting: Cell biology research laboratory. Methods: Clonogenic assays of EMT6 tumour cells with CDDP alone, CDDP plus simultaneous STS, and CDDP plus a 4-hour delay of STS were performed. Growth fractions under these three conditions were compared. Results: Tumour growth was statistically significantly increased when CDDP and STS were administered compared with CDDP alone. There was no statistically significant difference between simultaneous and 4-hour delay of STS administration. We conclude that in EMT6 cells, either simultaneous administration or a 4-hour delay of STS administration significantly decreases CDDP efficacy. Conclusion: STS adversely affects CDDP's oncologic efficacy in EMT6 cell cultures in vitro.

Original languageEnglish (US)
Pages (from-to)638-641
Number of pages4
JournalJournal of Otolaryngology - Head and Neck Surgery
Volume37
Issue number5
DOIs
StatePublished - Oct 1 2008

Keywords

  • Cancer
  • Chemotherapy
  • Cisplatin
  • Hair cell
  • Hearing
  • Ototoxicity
  • Sodium thiosulphate

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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