Delayed onset of brain edema and mislocalization of aquaporin-4 in dystrophin-null transgenic mice

Zsolt Vajda, Michael Pedersen, Ernst Martin Füchtbauer, Karin Wertz, Hans Stødkilde-Jørgensen, Endre Sulyok, Tamás Dóczi, John D. Neely, Peter C Agre, Jørgen Frøkiær, Søren Nielsen

Research output: Contribution to journalArticle

Abstract

Cerebral water accumulation was studied during induction of brain edema in dystrophin-null transgenic mice (mdx-βgeo) and control mice. Immunofluorescence and immunoelectron microscopic analyses of dystrophin-null brains revealed a dramatic reduction of AQP4 (aquaporin-4) in astroglial end-feet surrounding capillaries (blood-brain barrier) and at the glia limitans (cerebrospinal fluid-brain interface). The AQP4 protein is mislocalized, because immunoblotting showed that the total AQP4 protein abundance was unaltered. Brain edema was induced by i.p. injection of distilled water and 8-deamino-arginine vasopressin. Changes in cerebral water compartments were assessed by diffusion-weighted MRI with determination of the apparent diffusion coefficient (ADC). In dystrophin-null mice and control mice, ADC gradually decreased by 5-6% from baseline levels during the first 35 min, indicating the initial phase of intracellular water accumulation is similar in the two groups. At this point, the control mice sustained an abrupt, rapid decline in ADC to 58% ± 2.2% of the baseline at 52.5 min, and all of the animals were dead by 56 min. After a consistent delay, the dystrophin-null mice sustained a similar decline in ADC to 55% ± 3.4% at 66.5 min, when all of the mice were dead. These results demonstrate that dystrophin is necessary for polarized distribution of AQP4 protein in brain where facilitated movements of water occur across the blood-brain barrier and cerebrospinal fluid-brain interface. Moreover, these results predict that interference with the subcellular localization of AQP4 may have therapeutic potential for delaying the onset of impending brain edema.

Original languageEnglish (US)
Pages (from-to)13131-13136
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number20
DOIs
StatePublished - Oct 1 2002

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Aquaporin 4
Dystrophin
Brain Edema
Transgenic Mice
Water
Brain
Blood-Brain Barrier
Cerebrospinal Fluid
Water Movements
Deamino Arginine Vasopressin
Diffusion Magnetic Resonance Imaging
Immunoblotting
Neuroglia
Fluorescent Antibody Technique
Injections

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Vajda, Z., Pedersen, M., Füchtbauer, E. M., Wertz, K., Stødkilde-Jørgensen, H., Sulyok, E., ... Nielsen, S. (2002). Delayed onset of brain edema and mislocalization of aquaporin-4 in dystrophin-null transgenic mice. Proceedings of the National Academy of Sciences of the United States of America, 99(20), 13131-13136. https://doi.org/10.1073/pnas.192457099

Delayed onset of brain edema and mislocalization of aquaporin-4 in dystrophin-null transgenic mice. / Vajda, Zsolt; Pedersen, Michael; Füchtbauer, Ernst Martin; Wertz, Karin; Stødkilde-Jørgensen, Hans; Sulyok, Endre; Dóczi, Tamás; Neely, John D.; Agre, Peter C; Frøkiær, Jørgen; Nielsen, Søren.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 20, 01.10.2002, p. 13131-13136.

Research output: Contribution to journalArticle

Vajda, Z, Pedersen, M, Füchtbauer, EM, Wertz, K, Stødkilde-Jørgensen, H, Sulyok, E, Dóczi, T, Neely, JD, Agre, PC, Frøkiær, J & Nielsen, S 2002, 'Delayed onset of brain edema and mislocalization of aquaporin-4 in dystrophin-null transgenic mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 20, pp. 13131-13136. https://doi.org/10.1073/pnas.192457099
Vajda, Zsolt ; Pedersen, Michael ; Füchtbauer, Ernst Martin ; Wertz, Karin ; Stødkilde-Jørgensen, Hans ; Sulyok, Endre ; Dóczi, Tamás ; Neely, John D. ; Agre, Peter C ; Frøkiær, Jørgen ; Nielsen, Søren. / Delayed onset of brain edema and mislocalization of aquaporin-4 in dystrophin-null transgenic mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 20. pp. 13131-13136.
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abstract = "Cerebral water accumulation was studied during induction of brain edema in dystrophin-null transgenic mice (mdx-βgeo) and control mice. Immunofluorescence and immunoelectron microscopic analyses of dystrophin-null brains revealed a dramatic reduction of AQP4 (aquaporin-4) in astroglial end-feet surrounding capillaries (blood-brain barrier) and at the glia limitans (cerebrospinal fluid-brain interface). The AQP4 protein is mislocalized, because immunoblotting showed that the total AQP4 protein abundance was unaltered. Brain edema was induced by i.p. injection of distilled water and 8-deamino-arginine vasopressin. Changes in cerebral water compartments were assessed by diffusion-weighted MRI with determination of the apparent diffusion coefficient (ADC). In dystrophin-null mice and control mice, ADC gradually decreased by 5-6{\%} from baseline levels during the first 35 min, indicating the initial phase of intracellular water accumulation is similar in the two groups. At this point, the control mice sustained an abrupt, rapid decline in ADC to 58{\%} ± 2.2{\%} of the baseline at 52.5 min, and all of the animals were dead by 56 min. After a consistent delay, the dystrophin-null mice sustained a similar decline in ADC to 55{\%} ± 3.4{\%} at 66.5 min, when all of the mice were dead. These results demonstrate that dystrophin is necessary for polarized distribution of AQP4 protein in brain where facilitated movements of water occur across the blood-brain barrier and cerebrospinal fluid-brain interface. Moreover, these results predict that interference with the subcellular localization of AQP4 may have therapeutic potential for delaying the onset of impending brain edema.",
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AU - Vajda, Zsolt

AU - Pedersen, Michael

AU - Füchtbauer, Ernst Martin

AU - Wertz, Karin

AU - Stødkilde-Jørgensen, Hans

AU - Sulyok, Endre

AU - Dóczi, Tamás

AU - Neely, John D.

AU - Agre, Peter C

AU - Frøkiær, Jørgen

AU - Nielsen, Søren

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N2 - Cerebral water accumulation was studied during induction of brain edema in dystrophin-null transgenic mice (mdx-βgeo) and control mice. Immunofluorescence and immunoelectron microscopic analyses of dystrophin-null brains revealed a dramatic reduction of AQP4 (aquaporin-4) in astroglial end-feet surrounding capillaries (blood-brain barrier) and at the glia limitans (cerebrospinal fluid-brain interface). The AQP4 protein is mislocalized, because immunoblotting showed that the total AQP4 protein abundance was unaltered. Brain edema was induced by i.p. injection of distilled water and 8-deamino-arginine vasopressin. Changes in cerebral water compartments were assessed by diffusion-weighted MRI with determination of the apparent diffusion coefficient (ADC). In dystrophin-null mice and control mice, ADC gradually decreased by 5-6% from baseline levels during the first 35 min, indicating the initial phase of intracellular water accumulation is similar in the two groups. At this point, the control mice sustained an abrupt, rapid decline in ADC to 58% ± 2.2% of the baseline at 52.5 min, and all of the animals were dead by 56 min. After a consistent delay, the dystrophin-null mice sustained a similar decline in ADC to 55% ± 3.4% at 66.5 min, when all of the mice were dead. These results demonstrate that dystrophin is necessary for polarized distribution of AQP4 protein in brain where facilitated movements of water occur across the blood-brain barrier and cerebrospinal fluid-brain interface. Moreover, these results predict that interference with the subcellular localization of AQP4 may have therapeutic potential for delaying the onset of impending brain edema.

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