Delayed hypothermia preferentially increases expression of brain-derived neurotrophic factor exon III in rat hippocampus after asphyxial cardiac arrest

Peter Vosler, Eric S. Logue, Melissa J. Repine, Clifton W. Callaway

Research output: Contribution to journalArticle

Abstract

Brain-derived neurotrophic factor (BDNF) protein levels increase in rats treated with a regimen of delayed, mild hypothermia that improve neurological recovery after asphyxial cardiac arrest. BDNF transcription in rat brain involves at least five different BDNF exons (exons I-V) that produce four different varieties of mRNA, each containing exon V paired with one of exons I-IV. This study examined whether these different BDNF transcripts are differentially affected by cardiac arrest and by therapeutic hypothermia in rat hippocampus using a reverse transcription and PCR-based method. At 24 h after asphyxial cardiac arrest, transcripts containing exons I and III increased. In rats treated with hypothermia after cardiac arrest, transcripts containing exon III were further increased. No significant alterations in transcripts from exons II or IV were observed, though there was a trend for hypothermia to decrease message from these exons. These data suggest that hypothermia after cardiac arrest produces exon-specific changes in BDNF transcription.

Original languageEnglish (US)
Pages (from-to)21-29
Number of pages9
JournalMolecular Brain Research
Volume135
Issue number1-2
DOIs
StatePublished - Apr 27 2005
Externally publishedYes

Fingerprint

Brain-Derived Neurotrophic Factor
Heart Arrest
Hypothermia
Exons
Hippocampus
Induced Hypothermia
Nerve Growth Factors
Reverse Transcription
Polymerase Chain Reaction
Messenger RNA
Brain

Keywords

  • Brain derived neurotrophic factor
  • Hypothermia
  • Ischemia
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Delayed hypothermia preferentially increases expression of brain-derived neurotrophic factor exon III in rat hippocampus after asphyxial cardiac arrest. / Vosler, Peter; Logue, Eric S.; Repine, Melissa J.; Callaway, Clifton W.

In: Molecular Brain Research, Vol. 135, No. 1-2, 27.04.2005, p. 21-29.

Research output: Contribution to journalArticle

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