Abstract
Rat lenses stored for extended periods at -20°C showed degradation and eventual disappearance of vimentin. This may have been caused by the rupture of cells and/or organelles by growing ice crystals and the subsequent release and diffusion of proteases. Besides the disappearance of vimentin there was also a decrease in the intensity of the βB1 polypeptide after 3 months storage. The observations reported herein are generally qualitative in nature, but should serve to alert other investigators of a potential problem.
Original language | English (US) |
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Pages (from-to) | 219-223 |
Number of pages | 5 |
Journal | Ophthalmic Research |
Volume | 28 |
Issue number | 4 |
DOIs | |
State | Published - Jan 1 1996 |
Externally published | Yes |
Keywords
- Frozen storage
- Protein degradation
- Rat lens
- Vimentin
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience