Degradation of vimentin in frozen rat lenses

Frederick A. Bettelheim, J. Samuel Zigler

Research output: Contribution to journalArticlepeer-review

Abstract

Rat lenses stored for extended periods at -20°C showed degradation and eventual disappearance of vimentin. This may have been caused by the rupture of cells and/or organelles by growing ice crystals and the subsequent release and diffusion of proteases. Besides the disappearance of vimentin there was also a decrease in the intensity of the βB1 polypeptide after 3 months storage. The observations reported herein are generally qualitative in nature, but should serve to alert other investigators of a potential problem.

Original languageEnglish (US)
Pages (from-to)219-223
Number of pages5
JournalOphthalmic Research
Volume28
Issue number4
DOIs
StatePublished - Jan 1 1996

Keywords

  • Frozen storage
  • Protein degradation
  • Rat lens
  • Vimentin

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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