Degradation of vimentin in frozen rat lenses

Frederick A. Bettelheim; J. Samuel Zigler

doi:10.1159/000267906

Degradation of vimentin in frozen rat lenses

Frederick A. Bettelheim, J. Samuel Zigler

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Rat lenses stored for extended periods at -20°C showed degradation and eventual disappearance of vimentin. This may have been caused by the rupture of cells and/or organelles by growing ice crystals and the subsequent release and diffusion of proteases. Besides the disappearance of vimentin there was also a decrease in the intensity of the βB1 polypeptide after 3 months storage. The observations reported herein are generally qualitative in nature, but should serve to alert other investigators of a potential problem.

Original language	English (US)
Pages (from-to)	219-223
Number of pages	5
Journal	Ophthalmic Research
Volume	28
Issue number	4
DOIs	https://doi.org/10.1159/000267906
State	Published - Jan 1 1996
Externally published	Yes

Keywords

Frozen storage
Protein degradation
Rat lens
Vimentin

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1159/000267906

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title = "Degradation of vimentin in frozen rat lenses",

abstract = "Rat lenses stored for extended periods at -20°C showed degradation and eventual disappearance of vimentin. This may have been caused by the rupture of cells and/or organelles by growing ice crystals and the subsequent release and diffusion of proteases. Besides the disappearance of vimentin there was also a decrease in the intensity of the βB1 polypeptide after 3 months storage. The observations reported herein are generally qualitative in nature, but should serve to alert other investigators of a potential problem.",

keywords = "Frozen storage, Protein degradation, Rat lens, Vimentin",

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year = "1996",

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AU - Zigler, J. Samuel

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AB - Rat lenses stored for extended periods at -20°C showed degradation and eventual disappearance of vimentin. This may have been caused by the rupture of cells and/or organelles by growing ice crystals and the subsequent release and diffusion of proteases. Besides the disappearance of vimentin there was also a decrease in the intensity of the βB1 polypeptide after 3 months storage. The observations reported herein are generally qualitative in nature, but should serve to alert other investigators of a potential problem.

KW - Frozen storage

KW - Protein degradation

KW - Rat lens

KW - Vimentin

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Degradation of vimentin in frozen rat lenses

Abstract

Keywords

ASJC Scopus subject areas

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