Degradation of outer membrane protein A in Escherichia coli killing by neutrophil elastase

A. A. Belaaouaj, Sik Kim Kwang Sik Kim, S. D. Shapiro

Research output: Contribution to journalArticlepeer-review

Abstract

In determining the mechanism of neutrophil elastase (NE)-mediated killing of Escherichia coli, we found that NE degraded outer membrane protein A (OmpA), localized on the surface of Gram-negative bacteria. NE killed wild-type, but not OmpA-deficient, E. coli. Also, whereas NE-deficient mice had impaired survival in response to E. coli sepsis, as compared to wild-type mice, the presence or absence of NE had no influence on survival in response to sepsis that had been induced with OmpA-deficient E. coli. These findings define a mechanism of nonoxidative bacterial killing by NE and point to OmpA as a bacterial target in host defense.

Original languageEnglish (US)
Pages (from-to)1185-1187
Number of pages3
JournalScience
Volume289
Issue number5482
DOIs
StatePublished - Aug 18 2000
Externally publishedYes

ASJC Scopus subject areas

  • General

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