Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study

William S. Schleif; Robert S. Harlan; Frances Hamblin; Ernest K. Amankwah; Neil A. Goldenberg; Raquel G. Hernandez; Sara B. Johnson; Shannon Reed; David R. Graham

doi:10.1016/j.jpeds.2021.09.061

Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study

William S. Schleif, Robert S. Harlan, Frances Hamblin, Ernest K. Amankwah, Neil A. Goldenberg, Raquel G. Hernandez, Sara B. Johnson, Shannon Reed, David R. Graham

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Newborn screening using dried plasma spots offers preanalytical advantages over conventional cards for plasma-associated targets of interest. Herein we present dried plasma spot–based methods for measuring metabolites using a 250+ compound liquid chromatography tandem mass spectrometry library. Quality assurance reduced this library to 134, and from these, 30 compounds determined the normal newborn reference ranges.

Original language	English (US)
Pages (from-to)	251-256.e4
Journal	Journal of Pediatrics
Volume	241
DOIs	https://doi.org/10.1016/j.jpeds.2021.09.061
State	Published - Feb 2022

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health

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Schleif, W. S., Harlan, R. S., Hamblin, F., Amankwah, E. K., Goldenberg, N. A., Hernandez, R. G., Johnson, S. B., Reed, S., & Graham, D. R. (2022). Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study. Journal of Pediatrics, 241, 251-256.e4. https://doi.org/10.1016/j.jpeds.2021.09.061

Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study. / Schleif, William S.; Harlan, Robert S.; Hamblin, Frances et al.
In: Journal of Pediatrics, Vol. 241, 02.2022, p. 251-256.e4.

Research output: Contribution to journal › Article › peer-review

Schleif, WS, Harlan, RS, Hamblin, F, Amankwah, EK, Goldenberg, NA , Hernandez, RG , Johnson, SB, Reed, S & Graham, DR 2022, 'Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study', Journal of Pediatrics, vol. 241, pp. 251-256.e4. https://doi.org/10.1016/j.jpeds.2021.09.061

Schleif WS, Harlan RS, Hamblin F, Amankwah EK, Goldenberg NA , Hernandez RG et al. Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study. Journal of Pediatrics. 2022 Feb;241:251-256.e4. doi: 10.1016/j.jpeds.2021.09.061

Schleif, William S. ; Harlan, Robert S. ; Hamblin, Frances et al. / Defining the Healthy Infant Metabolome : Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study. In: Journal of Pediatrics. 2022 ; Vol. 241. pp. 251-256.e4.

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Defining the Healthy Infant Metabolome: Liquid Chromatography Tandem-Mass Spectrometry Analysis of Dried Blood Spot Extracts from the Prospective Research on Early Determinants of Illness and Children's Health Trajectories Birth Cohort Study

Abstract

ASJC Scopus subject areas

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