Defining single molecular forces required to activate integrin and Notch signaling

Xuefeng Wang, Taekjip Ha

Research output: Contribution to journalArticle

Abstract

Cell-cell and cell-matrix mechanical interactions through membrane receptors direct a wide range of cellular functions and orchestrate the development of multicellular organisms. To define the single molecular forces required to activate signaling through a ligand-receptor bond, we developed the tension gauge tether (TGT) approach in which the ligand is immobilized to a surface through a rupturable tether before receptor engagement. TGT serves as an autonomous gauge to restrict the receptor-ligand tension. Using a range of tethers with tunable tension tolerances, we show that cells apply a universal peak tension of about 40 piconewtons (pN) to single integrin-ligand bonds during initial adhesion. We find that less than 12 pN is required to activate Notch receptors. TGT can also provide a defined molecular mechanical cue to regulate cellular functions.

Original languageEnglish (US)
Pages (from-to)991-994
Number of pages4
JournalScience
Volume340
Issue number6135
DOIs
StatePublished - 2013

ASJC Scopus subject areas

  • General

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