Defective regulation of outwardly rectifying Cl- channels by protein kinase a corrected by insertion of CFTR

Marie Egan, Terence Flotte, Sandra Afione, Rikki Solow, Pamela L. Zeitlin, Barrie J. Carter, William B. Guggino

Research output: Contribution to journalArticlepeer-review

Abstract

CYSTIC fibrosis (CF) is a lethal genetic disease resulting in a reduced CI- permeability1, increased mucous sulphation 2, increased Na+ absorption3 and defective acidification of lysosomal vesicles4. The CF gene encodes a protein (the cystic fibrosis trans-membrane conductance regulator, CFTR5) that can function as a low-conductance Cl- channel with a linear current-voltage relationship whose regulation is defective in CF patients 6-8. Larger conductance, outwardly rectifying Cl- channels are also defective in CF and fail to activate when exposed either to cyclic AMP-dependent protein kinase A or to protein kinase C9-13. The role of the outwardly rectifying Cl- channel in CF has been questioned14. We report here that expression of recombinant CF genes using adeno-associated virus vectors in CF bronchial epithelial cells corrects defective Cl- secretion, that it induces the appearance of small, linear conductance Cl- channels, and restores protein kinase A activation of outwardly rectifying Cl- channels. These results re-establish an involvement of outwardly rectifying Cl- channels in CF and suggest that CFTR regulates more than one conductance pathway in airway tissues.

Original languageEnglish (US)
Pages (from-to)581-584
Number of pages4
JournalNature
Volume358
Issue number6387
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • General

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