TY - JOUR
T1 - Defective ceramide response in C3H/HeJ (Lpsd) macrophages
AU - Barber, Sheila A.
AU - Perera, Pin Yu
AU - Vogel, Stefanie N.
PY - 1995
Y1 - 1995
N2 - Lipid second messengers are gaining recognition as important mediators of extracellular signals. One such lipid, ceramide, generated from membrane sphingomyelin following stimulation with TNF-α, IL-1β, or IFN-γ, activates ceramide-activated kinase (CAK). A recent study demonstrated that LPS activated CAK without generating ceramide, suggesting that the LPS stimulation of cells mimics the second messenger function of ceramide. To compare ceramide to LPS signaling, we assessed the ability of LPS-responsive (Lpsn) and LPS-hyporesponsive (Lpsd) macrophages to respond directly to ceramide for enhanced expression of LPS-inducible genes. In contrast to macrophages from C3H/OuJ (Lpsn) mice, C3H/ HeJ (Lpsd) macrophages failed to respond to cell-permeable analogues of ceramide (C2,C6,C16) or sphingomyelinase. These results suggest that a common critical molecule, encoded by the LPs gene, regulates both ceramide and LPS signaling pathways.
AB - Lipid second messengers are gaining recognition as important mediators of extracellular signals. One such lipid, ceramide, generated from membrane sphingomyelin following stimulation with TNF-α, IL-1β, or IFN-γ, activates ceramide-activated kinase (CAK). A recent study demonstrated that LPS activated CAK without generating ceramide, suggesting that the LPS stimulation of cells mimics the second messenger function of ceramide. To compare ceramide to LPS signaling, we assessed the ability of LPS-responsive (Lpsn) and LPS-hyporesponsive (Lpsd) macrophages to respond directly to ceramide for enhanced expression of LPS-inducible genes. In contrast to macrophages from C3H/OuJ (Lpsn) mice, C3H/ HeJ (Lpsd) macrophages failed to respond to cell-permeable analogues of ceramide (C2,C6,C16) or sphingomyelinase. These results suggest that a common critical molecule, encoded by the LPs gene, regulates both ceramide and LPS signaling pathways.
UR - http://www.scopus.com/inward/record.url?scp=0029076213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029076213&partnerID=8YFLogxK
M3 - Article
C2 - 7650365
AN - SCOPUS:0029076213
SN - 0022-1767
VL - 155
SP - 2303
EP - 2305
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -