Decreased retinoylation in NIH 3T3 cells transformed with activated Ha-ras

Noriko Takahashi, Luigi M De Luca, Theodore R. Breitman

Research output: Contribution to journalArticle

Abstract

Retinoylation (retinoic acid acylation) is a posttranslation modification of proteins occurring in a variety of mammalian cell lines and in vivo. To gain further knowledge of the role of retinoylation we studied it in NIH 3T3 cells and NIH 3T3 cells transformed by an activated Ha-ras oncogene (NIH Ha-ras-3T3 cells). In serum-free medium retinoic acid (RA) inhibited growth of NIH 3T3 cells but did not inhibit growth of NIH Ha-ras-3T3 cells. After incubation with [3H]RA, the level of retinoylated protein in NIH 3T3 cells was about 1.5-fold greater than in NIH Ha-ras-3T3 cells. On one-dimensional polyacrylamide gel electrophoresis, both the rate and the extent of retinoylation were greater in NIH 3T3 cells. We detected about 40 retinoylated proteins in NIH 3T3 cells by two-dimensional polyacrylamide gel electrophoresis. Only about 15 proteins were retinoylated, but at reduced levels, in NIH Ha-ras-3T3 cells. These results suggest that the activated ras oncogene inhibits retinoylation. This inhibition may in turn be related to the loss of other RA responses of NIH 3T3 cells, including growth inhibition, retinoic acid catabolism, down-regulation of fibronectin biosynthesis, and induction of tissue-type transglutaminase, which are not seen to the same extent in NIH Ha-ras-3T3 cells.

Original languageEnglish (US)
Pages (from-to)80-84
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number1
DOIs
StatePublished - Oct 9 1997
Externally publishedYes

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NIH 3T3 Cells
Tretinoin
3T3 Cells
Electrophoresis
ras Genes
Proteins
Acylation
Biosynthesis
Serum-Free Culture Media
Growth
Cell growth
Fibronectins
Electrophoresis, Gel, Two-Dimensional
Cells
Polyacrylamide Gel Electrophoresis
Down-Regulation
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Decreased retinoylation in NIH 3T3 cells transformed with activated Ha-ras. / Takahashi, Noriko; De Luca, Luigi M; Breitman, Theodore R.

In: Biochemical and Biophysical Research Communications, Vol. 239, No. 1, 09.10.1997, p. 80-84.

Research output: Contribution to journalArticle

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