Decreased pulmonary alveolar macrophage bactericidal activity in splenectomized rats

H. Tien Lau, Mark A. Hardy, R. P. Altman

Research output: Contribution to journalArticle

Abstract

The organism most frequently encountered in postsplenectomy sepsis is Streptococcus pneumoniae, which is thought to invade via the upper respiratory tract. This study assesses the phagocytic and bactericidal activity of pulmonary alveolar macrophages (PAM) in normal, splenectomized, and one-third splenectomized autotransplanted rats at 8 weeks of age, 7 weeks post surgery. The PAMs from both the splenectomized and control rats all expressed the same ability to phagocytose latex beads and Fc-receptor-mediated phagocytosis of SRBC. In the bactericidal assay, bacterial growth, after 1 hr without macrophages, was 31 × 103 colony-forming units (CFU). In the presence of PAMs from splenectomized rats, the colony count was 22 × 103 (P <0.2). Bactericidal activity was highly evident when PAMs from control and autotransplanted rats were assayed, with CFU of 11.3 × 103 and 14.6 × 103, respectively (P <0.001, P <0.002, respectively). It is concluded that antibody-dependent and independent phagocytic activity of PAMs is unimpaired in splenectomized rats as compared to controls. The defect in rats splenectomized at 1 week of age resides in poor pneumococcal bactericidal activity of PAMs and is almost completely corrected by ip autotransplantation of one-third of the spleen.

Original languageEnglish (US)
Pages (from-to)568-571
Number of pages4
JournalJournal of Surgical Research
Volume34
Issue number6
DOIs
StatePublished - 1983
Externally publishedYes

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Alveolar Macrophages
Phagocytosis
Stem Cells
Fc Receptors
Autologous Transplantation
Streptococcus pneumoniae
Microspheres
Respiratory System
Sepsis
Spleen
Macrophages
Antibodies
Growth

ASJC Scopus subject areas

  • Surgery

Cite this

Decreased pulmonary alveolar macrophage bactericidal activity in splenectomized rats. / Tien Lau, H.; Hardy, Mark A.; Altman, R. P.

In: Journal of Surgical Research, Vol. 34, No. 6, 1983, p. 568-571.

Research output: Contribution to journalArticle

Tien Lau, H. ; Hardy, Mark A. ; Altman, R. P. / Decreased pulmonary alveolar macrophage bactericidal activity in splenectomized rats. In: Journal of Surgical Research. 1983 ; Vol. 34, No. 6. pp. 568-571.
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