Decreased mitochondrial DNA content in posttreatment salivary rinses from head and neck cancer patients

Wei Wen Jiang, Eli Rosenbaum, Elizabeth Mambo, Marianna Zahurak, Brett Masayesva, Andre Lopes Carvalho, Shaoyu Zhou, William H. Westra, Anthony J. Alberg, David Sidransky, Wayne Martin Koch, Joseph A. Califano

Research output: Contribution to journalArticle

Abstract

Purpose and Experimental Design: Alterations in mitochondrial DNA (mtDNA) sequence and content have been described in human tissues and tumors in association with smoking exposure. We did quantitative PCR analysis of cytochrome c oxidase (Cox) I and Cox II genes to measure changes in mtDNA content in pretreatment and posttreatment salivary rinses obtained from 76 patients undergoing surgical resection for primary head and neck squamous cell carcinoma. We also examined the relationship between changes in mtDNA content and postoperative radiation therapy, smoking exposure, alcohol intake, and other clinical characteristics. Results: Overall, mtDNA content in posttreatment saliva was significantly decreased. The mean change for Cox I was -0.21 [95% confidence interval (95% CI), -0.44 to 0.01, P = 0.06] and for Cox II was -0.31 (95% CI, -0.55 to -0.08, P = 0.01). Patients in the radiation therapy group exhibited a significant decrease compared with the nonradiated group (P = 0.03 for Cox I; P = 0.05 for Cox II). In addition, significant decreases in Cox I (-0.71; 95% CI, -1.17 to -0.25, P = 0.005) and Cox II (-0.65; 95% CI, -1.17 to -0.13, P = 0.02) were found in never-smoking patients but not in former or current smokers. Conclusion: Our data suggest that salivary mtDNA content is decreased in never smokers and in response to radiation therapy after primary surgical resection.

Original languageEnglish (US)
Pages (from-to)1564-1569
Number of pages6
JournalClinical Cancer Research
Volume12
Issue number5
DOIs
StatePublished - Mar 1 2006

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Head and Neck Neoplasms
Mitochondrial DNA
Oxidoreductases
Confidence Intervals
Radiotherapy
Smoking
Electron Transport Complex IV
Saliva
Research Design
Alcohols
Polymerase Chain Reaction
Genes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Jiang, W. W., Rosenbaum, E., Mambo, E., Zahurak, M., Masayesva, B., Carvalho, A. L., ... Califano, J. A. (2006). Decreased mitochondrial DNA content in posttreatment salivary rinses from head and neck cancer patients. Clinical Cancer Research, 12(5), 1564-1569. https://doi.org/10.1158/1078-0432.CCR-05-1471

Decreased mitochondrial DNA content in posttreatment salivary rinses from head and neck cancer patients. / Jiang, Wei Wen; Rosenbaum, Eli; Mambo, Elizabeth; Zahurak, Marianna; Masayesva, Brett; Carvalho, Andre Lopes; Zhou, Shaoyu; Westra, William H.; Alberg, Anthony J.; Sidransky, David; Koch, Wayne Martin; Califano, Joseph A.

In: Clinical Cancer Research, Vol. 12, No. 5, 01.03.2006, p. 1564-1569.

Research output: Contribution to journalArticle

Jiang, WW, Rosenbaum, E, Mambo, E, Zahurak, M, Masayesva, B, Carvalho, AL, Zhou, S, Westra, WH, Alberg, AJ, Sidransky, D, Koch, WM & Califano, JA 2006, 'Decreased mitochondrial DNA content in posttreatment salivary rinses from head and neck cancer patients', Clinical Cancer Research, vol. 12, no. 5, pp. 1564-1569. https://doi.org/10.1158/1078-0432.CCR-05-1471
Jiang, Wei Wen ; Rosenbaum, Eli ; Mambo, Elizabeth ; Zahurak, Marianna ; Masayesva, Brett ; Carvalho, Andre Lopes ; Zhou, Shaoyu ; Westra, William H. ; Alberg, Anthony J. ; Sidransky, David ; Koch, Wayne Martin ; Califano, Joseph A. / Decreased mitochondrial DNA content in posttreatment salivary rinses from head and neck cancer patients. In: Clinical Cancer Research. 2006 ; Vol. 12, No. 5. pp. 1564-1569.
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abstract = "Purpose and Experimental Design: Alterations in mitochondrial DNA (mtDNA) sequence and content have been described in human tissues and tumors in association with smoking exposure. We did quantitative PCR analysis of cytochrome c oxidase (Cox) I and Cox II genes to measure changes in mtDNA content in pretreatment and posttreatment salivary rinses obtained from 76 patients undergoing surgical resection for primary head and neck squamous cell carcinoma. We also examined the relationship between changes in mtDNA content and postoperative radiation therapy, smoking exposure, alcohol intake, and other clinical characteristics. Results: Overall, mtDNA content in posttreatment saliva was significantly decreased. The mean change for Cox I was -0.21 [95{\%} confidence interval (95{\%} CI), -0.44 to 0.01, P = 0.06] and for Cox II was -0.31 (95{\%} CI, -0.55 to -0.08, P = 0.01). Patients in the radiation therapy group exhibited a significant decrease compared with the nonradiated group (P = 0.03 for Cox I; P = 0.05 for Cox II). In addition, significant decreases in Cox I (-0.71; 95{\%} CI, -1.17 to -0.25, P = 0.005) and Cox II (-0.65; 95{\%} CI, -1.17 to -0.13, P = 0.02) were found in never-smoking patients but not in former or current smokers. Conclusion: Our data suggest that salivary mtDNA content is decreased in never smokers and in response to radiation therapy after primary surgical resection.",
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AU - Rosenbaum, Eli

AU - Mambo, Elizabeth

AU - Zahurak, Marianna

AU - Masayesva, Brett

AU - Carvalho, Andre Lopes

AU - Zhou, Shaoyu

AU - Westra, William H.

AU - Alberg, Anthony J.

AU - Sidransky, David

AU - Koch, Wayne Martin

AU - Califano, Joseph A.

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N2 - Purpose and Experimental Design: Alterations in mitochondrial DNA (mtDNA) sequence and content have been described in human tissues and tumors in association with smoking exposure. We did quantitative PCR analysis of cytochrome c oxidase (Cox) I and Cox II genes to measure changes in mtDNA content in pretreatment and posttreatment salivary rinses obtained from 76 patients undergoing surgical resection for primary head and neck squamous cell carcinoma. We also examined the relationship between changes in mtDNA content and postoperative radiation therapy, smoking exposure, alcohol intake, and other clinical characteristics. Results: Overall, mtDNA content in posttreatment saliva was significantly decreased. The mean change for Cox I was -0.21 [95% confidence interval (95% CI), -0.44 to 0.01, P = 0.06] and for Cox II was -0.31 (95% CI, -0.55 to -0.08, P = 0.01). Patients in the radiation therapy group exhibited a significant decrease compared with the nonradiated group (P = 0.03 for Cox I; P = 0.05 for Cox II). In addition, significant decreases in Cox I (-0.71; 95% CI, -1.17 to -0.25, P = 0.005) and Cox II (-0.65; 95% CI, -1.17 to -0.13, P = 0.02) were found in never-smoking patients but not in former or current smokers. Conclusion: Our data suggest that salivary mtDNA content is decreased in never smokers and in response to radiation therapy after primary surgical resection.

AB - Purpose and Experimental Design: Alterations in mitochondrial DNA (mtDNA) sequence and content have been described in human tissues and tumors in association with smoking exposure. We did quantitative PCR analysis of cytochrome c oxidase (Cox) I and Cox II genes to measure changes in mtDNA content in pretreatment and posttreatment salivary rinses obtained from 76 patients undergoing surgical resection for primary head and neck squamous cell carcinoma. We also examined the relationship between changes in mtDNA content and postoperative radiation therapy, smoking exposure, alcohol intake, and other clinical characteristics. Results: Overall, mtDNA content in posttreatment saliva was significantly decreased. The mean change for Cox I was -0.21 [95% confidence interval (95% CI), -0.44 to 0.01, P = 0.06] and for Cox II was -0.31 (95% CI, -0.55 to -0.08, P = 0.01). Patients in the radiation therapy group exhibited a significant decrease compared with the nonradiated group (P = 0.03 for Cox I; P = 0.05 for Cox II). In addition, significant decreases in Cox I (-0.71; 95% CI, -1.17 to -0.25, P = 0.005) and Cox II (-0.65; 95% CI, -1.17 to -0.13, P = 0.02) were found in never-smoking patients but not in former or current smokers. Conclusion: Our data suggest that salivary mtDNA content is decreased in never smokers and in response to radiation therapy after primary surgical resection.

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