Decreased L-type calcium current in antral smooth muscle cells of STZ-induced diabetic rats

S. Li, J. D.Z. Chen

Research output: Contribution to journalArticlepeer-review


Background: Diabetic gastroparesis (delayed gastric emptying) is associated with antral hypomotility. L-type Ca2+ channels play an important role in generation of action potentials and activation of contractions. This study was designed to investigate if the function of the L-type Ca2+ channels of antral circular smooth muscle cells (SMCs) is impaired in streptozotocin (STZ)-induced diabetic rats. Methods: Eight weeks after the injection of STZ or vehicle, whole-cell patch clamp was used to record Ca2+ currents, and isometric tension recording was used to measure Ca2+ influx-induced contractions in circular muscle strips. Solid gastric emptying was measured in diabetic and control rats. Protein expression of Ca2+αlC-subunit in antral smooth muscles was compared between diabetic and control rats. Key Results: (1) Solid gastric emptying, independent of age or bodyweight, was slower in the diabetic rats, even after acute correction of hyperglycemia. (2) Verapamil, a potent calcium channel blocker, dose dependently reduced solid gastric emptying in normal rats. (3) Current density of L-type Ca2+ channel at 10 mV in antral circular SMCs was significantly decreased in the diabetic rats (-9.8 ± 0.7 pA/pF vs -15.9 ± 1.0 pA/pF in control, p < 0.001). However, protein expression of the Ca2+ channel in antral muscles did not differ between diabetic and control rats. (4) Contractile responses to 1 and 3 mM [Ca2+] were significantly reduced in the diabetic antral circular muscle strips, indicative of reduced Ca2+ influx. Conclusions & Inferences: These data suggested that the decreased L-type Ca2+ current in antral SMCs may contribute to antral hypomotility in STZ-induced diabetic rats.

Original languageEnglish (US)
Pages (from-to)971-979
Number of pages9
JournalNeurogastroenterology and Motility
Issue number7
StatePublished - Jul 2014


  • Calcium channel
  • Calcium channel blocker
  • Diabetes
  • Gastric emptying
  • L-type
  • Smooth muscle cell

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology


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