Decreased intraplatelet Ca2+ release and ATP secretion in pediatric nephrotic syndrome

Stanislav I. Svetlov, Elena S. Moskaleva, Vsevolod G. Pinelis, Yevgeny Daikhin, Victor L. Serebruany

Research output: Contribution to journalArticle

Abstract

Platelets play an important role in the natural history of idiopathic nephrotic syndrome (NS). Although thromboembolic events are rare, the activation of circulating platelets is generally considered an important factor in the prethrombotic state in children with NS. Platelet-activating factor (PAF), a potent endogenous phospholipid mediator of inflammation, stimulates intracellular free calcium (Ca2+) mobilization, aggregation, and release reactions in platelets obtained from normal donors. Platelet-related effects of PAF in children with NS are unknown. We studied PAF-induced intracellular Ca2+ mobilization in washed platelets and ATP secretion in platelet-rich plasma in 34 children with idiopathic NS and in 7 healthy children. There was a significant decrease in ATP secretion: 0.021 ± 0.011 μg/107 cells with 20 nM PAF and 0.089 ± 0.017 μg/107 platelets with 200 nM PAF versus control values (0.195 ± 0.004 μg/107 and 0.813 ± 0.09 μg/107, respectively). Moreover, PAF-evoked increase in intracellular free Ca2+ concentration was twofold lower in NS patients than in control subjects (230.1 ± 22.4 nM versus 455.6 ± 15.3 nM). Also, thrombin-induced intracellular free Ca2+ mobilization was diminished in children with NS compared with the control group. Thus, contrary to expectations, a decrease of platelet reactivity in response to PAF in vitro was observed in children with idiopathic NS. We suggest that this decreased platelet reactivity may reflect a period refractory to PAF and may be considered as platelet desensitization to PAF released in vivo in children with NS.

Original languageEnglish (US)
Pages (from-to)205-208
Number of pages4
JournalPediatric Nephrology
Volume13
Issue number3
DOIs
StatePublished - Apr 1999

Fingerprint

Platelet Activating Factor
Nephrotic Syndrome
Adenosine Triphosphate
Blood Platelets
Pediatrics
Inflammation Mediators
Platelet-Rich Plasma
Platelet Activation
Natural History
Thrombin
Phospholipids
Tissue Donors
Calcium
Control Groups

Keywords

  • ATP release
  • Calcium mobilization
  • Nephrotic syndrome
  • Platelet-activating factor
  • Platelets

ASJC Scopus subject areas

  • Nephrology
  • Pediatrics, Perinatology, and Child Health

Cite this

Svetlov, S. I., Moskaleva, E. S., Pinelis, V. G., Daikhin, Y., & Serebruany, V. L. (1999). Decreased intraplatelet Ca2+ release and ATP secretion in pediatric nephrotic syndrome. Pediatric Nephrology, 13(3), 205-208. https://doi.org/10.1007/s004670050593

Decreased intraplatelet Ca2+ release and ATP secretion in pediatric nephrotic syndrome. / Svetlov, Stanislav I.; Moskaleva, Elena S.; Pinelis, Vsevolod G.; Daikhin, Yevgeny; Serebruany, Victor L.

In: Pediatric Nephrology, Vol. 13, No. 3, 04.1999, p. 205-208.

Research output: Contribution to journalArticle

Svetlov, SI, Moskaleva, ES, Pinelis, VG, Daikhin, Y & Serebruany, VL 1999, 'Decreased intraplatelet Ca2+ release and ATP secretion in pediatric nephrotic syndrome', Pediatric Nephrology, vol. 13, no. 3, pp. 205-208. https://doi.org/10.1007/s004670050593
Svetlov, Stanislav I. ; Moskaleva, Elena S. ; Pinelis, Vsevolod G. ; Daikhin, Yevgeny ; Serebruany, Victor L. / Decreased intraplatelet Ca2+ release and ATP secretion in pediatric nephrotic syndrome. In: Pediatric Nephrology. 1999 ; Vol. 13, No. 3. pp. 205-208.
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