Decreased collagen production in chronologically aged skin: Roles of age-dependent alteration in fibroblast function and defective mechanical stimulation

James Varani, Michael K. Dame, Laure Rittie, Suzanne E G Fligiel, Sewon Kang, Gary J. Fisher, John J. Voorhees

Research output: Contribution to journalArticle

Abstract

Reduced synthesis of collagen types I and III is characteristic of chronologically aged skin. The present report provides evidence that both cellular fibroblast aging and defective mechanical stimulation in the aged tissue contribute to reduced collagen synthesis. The reduction in collagen synthesis due to fibroblast aging was demonstrated by a lower in vitro production of type I procollagen by dermal fibroblasts isolated from skin of young (18 to 29 years) versus old (80+ years) individuals (82 ± 16 versus 56 ± 8 ng/ml; P <0.05). A reduction in mechanical stimulation in chronologically aged skin was inferred from morphological, ultrastructural, and fluorescence microscopic studies. These studies, comparing dermal sections from young and old individuals, demonstrated a greater percentage of the cell surface attached to collagen fibers (78 ± 6 versus 58 ± 8%; P <0.01) and more extensive cell spreading (1.0 ± 0.3 vs. 0.5 ± 0.3; P <0.05) in young skin compared with old skin. These features are consistent with a lower level of mechanical stimulation on the cells in old versus young skin. Based on the findings presented here, we conclude that reduced collagen synthesis in chronologically aged skin reflects at least two different underlying mechanisms: cellular fibroblast aging and a lower level of mechanical stimulation.

Original languageEnglish (US)
Pages (from-to)1861-1868
Number of pages8
JournalAmerican Journal of Pathology
Volume168
Issue number6
DOIs
StatePublished - Jun 2006
Externally publishedYes

Fingerprint

Collagen
Fibroblasts
Skin
Cell Aging
Collagen Type I
Collagen Type III
Fluorescence

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Decreased collagen production in chronologically aged skin : Roles of age-dependent alteration in fibroblast function and defective mechanical stimulation. / Varani, James; Dame, Michael K.; Rittie, Laure; Fligiel, Suzanne E G; Kang, Sewon; Fisher, Gary J.; Voorhees, John J.

In: American Journal of Pathology, Vol. 168, No. 6, 06.2006, p. 1861-1868.

Research output: Contribution to journalArticle

Varani, James ; Dame, Michael K. ; Rittie, Laure ; Fligiel, Suzanne E G ; Kang, Sewon ; Fisher, Gary J. ; Voorhees, John J. / Decreased collagen production in chronologically aged skin : Roles of age-dependent alteration in fibroblast function and defective mechanical stimulation. In: American Journal of Pathology. 2006 ; Vol. 168, No. 6. pp. 1861-1868.
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