Deconvolution of the cellular force-generating subsystems that govern cytokinesis furrow ingression

Christopher C. Poirier, Win Pin Ng, Douglas Robinson, Pablo A Iglesias

Research output: Contribution to journalArticle

Abstract

Cytokinesis occurs through the coordinated action of several biochemically-mediated stresses acting on the cytoskeleton. Here, we develop a computational model of cellular mechanics, and using a large number of experimentally measured biophysical parameters, we simulate cell division under a number of different scenarios. We demonstrate that traction-mediated protrusive forces or contractile forces due to myosin II are sufficient to initiate furrow ingression. Furthermore, we show that passive forces due to the cell's cortical tension and surface curvature allow the furrow to complete ingression. We compare quantitatively the furrow thinning trajectories obtained from simulation with those observed experimentally in both wild-type and myosin II null Dictyostelium cells. Our simulations highlight the relative contributions of different biomechanical subsystems to cell shape progression during cell division.

Original languageEnglish (US)
Article numbere1002467
JournalPLoS Computational Biology
Volume8
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

Myosin Type II
Cytokinesis
cytokinesis
Deconvolution
deconvolution
furrows
Cell Division
Subsystem
Myosin
Cells
myosin
Null Lymphocytes
traction (mechanics)
Dictyostelium
Surface Tension
Cell Shape
cell division
Cell
Traction
Mechanics

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Ecology
  • Molecular Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Computational Theory and Mathematics

Cite this

Deconvolution of the cellular force-generating subsystems that govern cytokinesis furrow ingression. / Poirier, Christopher C.; Ng, Win Pin; Robinson, Douglas; Iglesias, Pablo A.

In: PLoS Computational Biology, Vol. 8, No. 4, e1002467, 04.2012.

Research output: Contribution to journalArticle

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