Deconstructing and constructing innate immune functions using molecular sensors and actuators

Kester Coutinho, Takanari Inoue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

White blood cells such as neutrophils and macrophages are made competent for chemotaxis and phagocytosis - the dynamic cellular behaviors that are hallmarks of their innate immune functions - by the reorganization of complex biological circuits during differentiation. Conventional loss-of-function approaches have revealed that more than 100 genes participate in these cellular functions, and we have begun to understand the intricate signaling circuits that are built up from these gene products. We now appreciate: (1) that these circuits come in a variety of flavors - so that we can make a distinction between genetic circuits, metabolic circuits and signaling circuits; and (2) that they are usually so complex that the assumption of multiple feedback loops, as well as that of crosstalk between seemingly independent pathways, is now routine. It has not escaped our notice, however, that just as physicists and electrical engineers have long been able to disentangle complex electric circuits simply by repetitive cycles of probing and measuring electric currents using a voltmeter, we might similarly be able to dissect these intricate biological circuits by incorporating equivalent approaches in the fields of cell biology and bioengineering. Existing techniques in biology for probing individual circuit components are unfortunately lacking, so that the overarching goal of drawing an exact circuit diagram for the whole cell - complete with kinetic parameters for connections between individual circuit components - is not yet in near sight. My laboratory and others have thus begun the development of a new series of molecular tools that can measurably investigate the circuit connectivity inside living cells, as if we were doing so on a silicon board. In these proceedings, I will introduce some of these techniques, provide examples of their implementation, and offer a perspective on directions moving forward.

Original languageEnglish (US)
Title of host publicationSensing and Analysis Technologies for Biomedical and Cognitive Applications 2016
PublisherSPIE
Volume9871
ISBN (Electronic)9781510601123
DOIs
StatePublished - 2016
EventSensing and Analysis Technologies for Biomedical and Cognitive Applications 2016 - Baltimore, United States
Duration: Apr 17 2016Apr 18 2016

Other

OtherSensing and Analysis Technologies for Biomedical and Cognitive Applications 2016
CountryUnited States
CityBaltimore
Period4/17/164/18/16

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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