DeCoN: Genome-wide analysis of invivo transcriptional dynamics during pyramidal neuron fate selection in neocortex

Bradley J. Molyneaux, Loyal A. Goff, Andrea C. Brettler, Hsu Hsin Chen, Juliana R. Brown, Siniša Hrvatin, John L. Rinn, Paola Arlotta

Research output: Contribution to journalArticle

Abstract

Neuronal development requires a complex choreography of transcriptional decisions to obtain specific cellular identities. Realizing the ultimate goal of identifying genome-wide signatures that define and drive specific neuronal fates has been hampered by enormous complexity in both time and space during development. Here, we have paired high-throughput purification of pyramidal neuron subclasses with deep profiling of spatiotemporal transcriptional dynamics during corticogenesis to resolve lineage choice decisions. We identified numerous features ranging from spatial and temporal usage of alternative mRNA isoforms and promoters to a host of mRNA genes modulated during fate specification. Notably, we uncovered numerous long noncoding RNAs with restricted temporal and cell-type-specific expression. To facilitate future exploration, we provide an interactive online database to enable multidimensional data mining and dissemination. This multifaceted study generates a powerful resource and informs understanding of the transcriptional regulation underlying pyramidal neuron diversity in the neocortex.

Original languageEnglish (US)
Pages (from-to)275-288
Number of pages14
JournalNeuron
Volume85
Issue number2
DOIs
StatePublished - Jan 21 2015

ASJC Scopus subject areas

  • Neuroscience(all)

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    Molyneaux, B. J., Goff, L. A., Brettler, A. C., Chen, H. H., Brown, J. R., Hrvatin, S., Rinn, J. L., & Arlotta, P. (2015). DeCoN: Genome-wide analysis of invivo transcriptional dynamics during pyramidal neuron fate selection in neocortex. Neuron, 85(2), 275-288. https://doi.org/10.1016/j.neuron.2014.12.024